| 加拿大进境油菜籽茎基溃疡病菌的检测与鉴定 |
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| 引用本文： | 王振华,杨 武,赵 晖,曾宪东,李凤新,蔡 翔,王瀚昌,余 浩. 加拿大进境油菜籽茎基溃疡病菌的检测与鉴定[J]. 华中农业大学学报, 2011, 30(1): 66-69 |
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| 作者姓名： | 王振华 杨 武 赵 晖 曾宪东 李凤新 蔡 翔 王瀚昌 余 浩 |
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| 作者单位： | 1. 湖北出入境检验检疫局,武汉,430050
2. 华中农业大学植物科技学院,武汉,430070 |
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| 摘 要： | 从加拿大进境油菜籽中随机选取船载油菜籽样品,通过特异引物的PCR扩增检测和病原菌分离鉴定,观察了油菜茎基溃疡病菌Leptosphaeria maculans的发生情况.结果表明:所有来自3艘轮船的15份油菜籽样品的油菜茎基溃疡病菌PCR检测都呈阳性;随机选择的3份阳性样品的病原菌分离、鉴定与致病性测试结果证实了PCR的...
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| 关 键 词： | 油菜茎基溃疡病菌 检测 鉴定 |
| 收稿时间： | 2010-06-10 |
Detection and identification of Leptosphaeria maculans in imported Canadian rapeseeds |
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| WANG Zhen-hua,YANG Wu,ZHAO Hui,ZENG Xian-dong,LI Feng-xin,CAI Xiang,WANG Han-chang,YU Hao. Detection and identification of Leptosphaeria maculans in imported Canadian rapeseeds[J]. Journal of Huazhong Agricultural University, 2011, 30(1): 66-69 |
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| Authors: | WANG Zhen-hua YANG Wu ZHAO Hui ZENG Xian-dong LI Feng-xin CAI Xiang WANG Han-chang YU Hao |
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| Affiliation: | WANG Zhen-hua1 YANG Wu1 ZHAO Hui1 ZENG Xian-dong1 LI Feng-xin1 CAI Xiang2 WANG Han-chang1 YU Hao11.Hubei Entry-Exit Inspection and Quarantine Bureau of P.R.China,Wuhan 430050,China,2.College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070 |
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| Abstract: | In this research,shipborne samples were randomly selected from imported Canadian rapeseeds to investigate the occurrence of phoma stem canker(Leptosphaeria maculans) by PCR amplification,pathogen isolation and pathogenicity test.The results showed that all fifteen rapeseed samples from three ships were positive in PCR detection of Leptosphaeria maculans.The results from pathogen isolation,identification and pathogenicity testing of three randomly selected samples confirmed the PCR detection. |
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| Keywords: | Leptosphaeria maculans detection identification |
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