蓖麻种质资源遗传多样性与群体结构的SSR分析

为明确蓖麻种质资源的遗传多样性和群体遗传结构,利用SSR分子标记结合PopGene1.32软件、MEGA7.0软件和Structure2.3.4软件等,对来源于国内5个地区(16个省市)和国外3个国家的191份蓖麻种质进行遗传多样性和群体遗传结构分析.结果表明:86对SSR引物共检测出194个等位变异,其中平均等位基因...

Genetic diversity and population structure analysis of castor bean accessions based on SSR markers

To better understand the genetic diversity and population structure of castor bean germplasm resources, SSR marker analysis of 191 castor bean accessions from 5 areas (including 16 provinces or municipalities) of China and other 3 countries was performed with softwares PopGene1.32, MEGA7.0 and Structure2.3.4. It was showed that 194 polymorphic alleles were detected by 86 SSR primers, with average allele number(Na)of 2.26.The average heterozygosity(Ho)and expected heterozygosity (He) were 0.29 and 0.36 respectively. Shannon diversity index (I) was 0.54. Based on cluster analysis, the 191 accessions were assigned into 2 groups at the genetic distance of 0.20, each group contained germplasms from different origin areas. No directly relation was found between genetic distance and geographic origin. These accessions were also divided into 2 clusters by Structure2.3.4 software, that was similar to the cluster result. However, Q value of 170 accessions were higher than 0.6, suggesting a relatively single blood relationship. Another 21 accessions had mixed pedigree with complicated genetic background. The above results was expected to provide basis for genetic background, breeding improvement and parent selection in castor bean.