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| 银狐生长激素成熟mRNA真核表达载体的构建 | |
| 引用本文： | 胡晓航,闫丽辉,杨传平,曹殿军,刘培欣,刘玉堂. 银狐生长激素成熟mRNA真核表达载体的构建[J]. 东北林业大学学报, 2005, 33(3): 102-103 |
| 作者姓名： | 胡晓航 闫丽辉 杨传平 曹殿军 刘培欣 刘玉堂 |
| 作者单位： | 1. 东北林业大学,哈尔滨,150040 2. 中国农业科学院哈尔滨兽医研究所 3. 东北林业大学 |
| 摘 要： | 用PCR方法，从含有银狐生长激素mRNA的阳性重组质粒pMD18-T-IGH中亚克隆出生长激素基因，然后定向插入到真核表达载体pcDNA3．1( )中，通过酶切、质粒PCR和序列测定对质粒进行鉴定表明，成功构建了银狐生长激素成熟mRNA真核表达质粒pcDNA3．1-IGH． |
| 关 键 词： | 银狐 生长激素 真核表达载体 |
Construction and Identification of Eukaryotic Expression Vector of Growth Hormone of Vulpes vulpe |
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| Hu Xiaohang,YAN Lihui,Yang Chuanping,CAO Dianjun,LIU Peixin,Liu Yutang. Construction and Identification of Eukaryotic Expression Vector of Growth Hormone of Vulpes vulpe[J]. Journal of Northeast Forestry University, 2005, 33(3): 102-103 | |
| Authors: | Hu Xiaohang YAN Lihui Yang Chuanping CAO Dianjun LIU Peixin Liu Yutang |
| Abstract: | Growth hormone gene of Vulpes vulpes was sub-cloned by polymerase chain reaction from recombined pMD18-T-GHcDNA plasmid, and then inserted into eukaryotic expressed vector pcDNA3.1. It was proved by restrict enzyme analysis and sequencing. The result of identification showed that eukaryotic expression vector pcDNA3.1-GHcDNA was successfully constructed. |
| Keywords: | mRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |