| 甘蓝型油菜杂交种亲本指纹图谱分析 |
| |
| 引用本文： | 段院生,刘平武,杨光圣. 甘蓝型油菜杂交种亲本指纹图谱分析[J]. 中国油料作物学报, 2002, 24(2): 5-9 |
| |
| 作者姓名： | 段院生 刘平武 杨光圣 |
| |
| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,国家油菜改良武汉分中心,湖北,武汉,430070 |
| |
| 基金项目： | 国家 8 63项目 (2 0 0 1AA2 41111) |
| |
| 摘 要： | 用140个随机引物对39份甘蓝型双低油菜杂交种亲本进行RAPD分析，选出40个能产生清晰多态性主带的引物重复扩增，以筛选出的9个引的扩增出重复性好的多态性主带构建39份亲本材料的DNA指纹图谱。结果表明，DNA指纹图谱可将27份亲本材料一一区分，其余12份材料可合并为4组，组内材料均为来源相同的姊妹系。指纹图谱鉴定结果与材料实际来源相吻合。
|
| 关 键 词： | 甘蓝型油菜 杂种亲本 RAPD 指纹图谱分析 |
| 文章编号： | 1007-9084(2002)02-0005-05 |
| 修稿时间： | 2002-05-30 |
Fingerprinting analysis of hybrid parents in Brassica napus based on RAPD |
| |
| DUAN Yuan-sheng,LIU Ping-wu,YANG Guang-sheng. Fingerprinting analysis of hybrid parents in Brassica napus based on RAPD[J]. Chinese Journal of Oil Crop Sciences, 2002, 24(2): 5-9 |
| |
| Authors: | DUAN Yuan-sheng LIU Ping-wu YANG Guang-sheng |
| |
| Abstract: | random primers were used for PCR amplifications with 39 hybrid parents in Brassica napus , and 40 primers were chosen to further PCR amplifications for them producing main bands with good repeatility. Finally 9 best primers were selected for fingerprinting analysis in this study. The fingerprints obtained from these 9 primers could distinguish 27 materials and the other four groups consisting of 12 materials. The materials in each group were sister materials. The fingerprints of these 39 materials were consistent with their pedigree. |
| |
| Keywords: | Brassica napus Hybrid parent DNA fingerprint Random amplified polymorphic DNA (RAPD) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
