河南省豫西地区农田土壤镰孢菌种群多样性分析

采用形态学和现代分子生物学方法,对河南省豫西地区农田土壤镰孢菌种群多样性进行了分析。通过鉴定,共得到了9个种的镰孢菌,分别为尖孢镰孢菌、半裸镰孢菌、轮枝镰孢菌、禾谷镰孢菌、层生镰孢菌、锐顶镰孢菌、黄色镰孢菌、木贼镰孢菌和茄腐镰孢菌。系统发育学分析结果表明:镰孢菌属真菌18S rDNA-ITS和EF-1α基因序列均具有一定的同源性和丰富的遗传多态性,其中李瑟组镰孢菌轮枝镰孢菌、层生镰孢菌、黄色镰孢菌种间18S rDNA-ITS基因序列存在着较高的同源性,不能正确地表现镰孢菌属种一级分类单元的特征;而延伸因子EF-1α基因序列却较好地表现出了供试菌株种一级的关系,说明镰孢菌延伸因子EF-1α基因序列较18S rDNA-ITS基因序列存在更丰富的种间多态性,可以较好地表现镰孢菌种一级的关系。

Analysis of Fusarium Species Diversity from Field Soil in Western Henan Province

It was analyzed the phylogenetic relationships among Fusarium strains for the soil diversity of Fusarium species in western Henan province.The species diversity on Fusarium of field soil in western Henan province was analyzed by methods of morphological modern molecular biology.It was obtained nine species of Fusarium through the identification.There are F.oxysporum,F.semitectum,F.verticillioides,F.graminearum,F.proliferatum,F.acumininatum,F.equiseti,F.thapsinum and F.solani.Phylogenetic analysis showed that 18S rDNA-ITS and EF-1α gene sequences showed homology and the genetic polymorphism.18S rDNA-ITS gene sequence analysis showed that F verticillioides,F.proliferatum and F.thapsinum which belonged to Liseola Fusarium fungi species presenced high homology.The performance can not be correct to manifest the classification of the Fusarium species.Elongation factor EF-1α gene sequences better demonstrated the relationship of the tested strains between the species level.It indicated that elongation factor EF-1α gene sequences exist more abundant species polymorphisms than 18S rDNA-ITS gene sequence.It can be more better to show the relationship between the level of Fusarium species.