|
|||||
|
|
| 绵羊卵母细胞体外培养Ghrelin mRNA的表达 | |
| 引用本文： | 杜晨光,彭春霞,刘永斌,王昆.绵羊卵母细胞体外培养Ghrelin mRNA的表达[J].华北农学报,2012,27(2):81-84. |
| 作者姓名： | 杜晨光 彭春霞 刘永斌 王昆 |
| 作者单位： | 1. 内蒙古农业大学职业技术学院,内蒙古包头014109;河北省农林科学院粮油作物研究所,河北石家庄050035 2. 乌兰浩特市动物疫病预防控制中心,内蒙古乌兰浩特,137400 3. 内蒙古赛科星繁育生物技术股份有限公司,内蒙古呼和浩特,011517 4. 河北省农林科学院粮油作物研究所,河北石家庄,050035 |
| 基金项目： | 内蒙古自然科学基金，内蒙人才基金，内蒙古高等学校科学研究项目，内蒙古农业大学职业技术学院应用技术研究基金 |
| 摘 要： | 为揭示Ghrelin在绵羊卵母细胞的表达情况,运用实时定量RT-PCR技术检测不同培养系统中绵羊卵母细胞Ghrelin mRNA的相对表达量。结果表明,以M199和血清为基础培养基添加不同激素后培养的卵母细胞GhrelinmRNA相对表达量均显著上升,仅以M199为基础培养基添加不同激素后培养的卵母细胞Ghrelin mRNA相对表达量均显著下降。在不同基础培养基中添加不同激素后,E2较FSH和LH有促进Ghrelin mRNA相对表达量升高的趋势。绵羊卵母细胞Ghrelin mRNA的表达以及在不同培养系统动力学变化,提示了这一新型分子在卵母细胞成熟过程中受激素潜在的调控作用。 |
| 关 键 词： | Ghrelin 卵母细胞 体外培养 实时定量RT-PCR |
Expression of Ghrelin mRNA in the Ovine Oocytes During in vitro Different Culture System |
|
| DU Chen-guang , PENG Chun-xia , LIU Yong-bin , WANG Kun.Expression of Ghrelin mRNA in the Ovine Oocytes During in vitro Different Culture System[J].Acta Agriculturae Boreali-Sinica,2012,27(2):81-84. | |
| Authors: | DU Chen-guang PENG Chun-xia LIU Yong-bin WANG Kun |
| Institution: | 1.Vocational and Technical College,Inner Mongolia Agricultural University,Baotou 014109,China;2.Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031,China;3.Wulanhot for Center for Animal Disease Control and Prevention,Wulanhot 137400,China;4.Inner Mongolia Saikexing Reproductive Biotechnology Co.Ltd,Huhhot 011517,China) |
| Abstract: | Relative real-time RT-PCR experiments confirmed that the Ghrelin mRNA levels varied depending on the different culture systems,with the significantly high expression in the maturation base on the serum,whereas the oocytes significantly decline without serum.The levels of Ghrelin mRNA decreased affected by FSH and LH,the E2 showed promoting the Ghrelin mRNA both media.Dynamic changes and the persistent presence of the Ghrelin signaling system within oocytes open up the possibility of a potential regulatory role by hormones of this novel molecule in reproductive function. |
| Keywords: | Ghrelin Oocyte In vitro culture Real time RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |