黄瓜黄色子叶突变体遗传及相关AFLP标记

以黄色子叶突变体B180H为母本,颜色正常子叶黄瓜Q12为父本,配制F1、F2、BC1,并通过对6世代子叶叶色特征的观察和统计分析,研究该黄色子叶性状的遗传规律,并利用BSA法、AFLP技术筛选与黄色子叶性状基因紧密连锁的分子标记。结果表明,黄瓜黄色子叶性状是由1对核基因控制的稳定遗传的隐性性状,绿色子叶相对于黄色为完全显性。通过BSA法和AFLP分子标记,应用1 024对引物组合EcoRⅠ-NN/MseⅠ-NNN对黄色、绿色子叶亲本进行筛选,筛选到了1个与黄瓜黄色子叶性状相关的共显性标记E-AG/M-AAG。测序结果表明,片段长度分别为258 bp和257 bp,为发生插入/缺失或突变的同源序列;在黄色子叶个体中只扩增出了258 bp的特异片段,绿色子叶个体中扩增出了257 bp的特异片段或同时扩增出258 bp和257 bp两个特异片段。经组外其他F2单株验证发现,鉴定结果符合率高达96.74%,以Mapmaker 3.0软件分析,该标记与子叶黄色突变位点的连锁距离为3.2 cM。该性状可作为苗期遗传标记性状,在杂交育种和品种纯度鉴定上有极大的利用价值。

Genetic Analysis and AFLP Markers Associated to the Yellow Cotyledon Trait in Cucumber

With a F2 and BC1 population derived from F1 of B180H(yellow cotyledon female)and Q12(green cotyledon male)was used as materials to study the inheritance of yellow cotyledon color and molecular marker associated to the cotyledon yellow trait.The results indicated that the yellow cotyledon trait was controlled by a pair of recessive gene,and the green cotyledon trait was in the mode of incomplete dominant inheritance.The polymorphism between yellow and green cotyledon parents of cucumber were studied using BSA method and AFLP technology.1 024 AFLP markers were screened,and a co-dominant marker E-AG/M-AAG was obtained.Sequencing of the polymorphic fragment indicated that their lengths were 258 bp and 257 bp,respectively,and they were orthologous sequences with an insertion /deletion or mutant of only one nucleotide.The yellow cotyledon F2 plant possessed the 258 bp fragments,and the green cotyledon F2 plants possessed the 257 bp fragments or possessed both two fragments.The marker was closely linked to the cucumber cotyledon yellow color trait-related gene,and the marker was confirmed to be 96.74% accurate by F2 individuals.The trait was a useful marker for hybridization and purity identification of F1 hybrids.