CO2加富下空气湿度调控对高温大棚嫁接黄瓜逆境生理的影响

试验在CO2加富,气温为40～45℃条件下,将空气湿度设置为(95±5)%(L1),(75±5)%(L2)和(55±5)%(L3)3个处理,研究了空气湿度调控对大棚嫁接黄瓜逆境生理的影响,以期为大棚黄瓜越夏高产栽培奠定理论基础。结果表明:随着处理时间的延长,3个处理黄瓜叶片中的渗透调节物质均呈增加趋势,即丙二醛含量L2,L3呈增加趋势,而L1则呈先增后降趋势变化;细胞膜透性L2,L3呈增加趋势,L1则呈降低趋势变化;保护酶活性L1,L2呈增强趋势,而L3则呈先增后降趋势变化。不同的空气湿度处理中,随着处理湿度的升高,黄瓜叶片中的渗透调节物质、丙二醛含量、保护酶活性和细胞膜透性均呈降低趋势,表现为L1﹤L2﹤L3,在处理第24天时,L1比L2,L3的可溶性蛋白含量分别降低了17.23%和18.61%,脯氨酸含量分别降低了51.28%和70.72%,可溶性糖含量分别降低了24.02%和37.21%,丙二醛含量分别降低了43.44%和55.28%,细胞膜透性分别降低了74.46%和74.46%,超氧化物歧化酶(SOD)活性分别降低了41.88%和38.19%,过氧化氢酶(CAT)活性分别降低了61.21%和67.35%,过氧化物酶(POD)活性分别降低了27.80%和39.45%。说明在CO2加富和空气湿度为(95±5)%处理下,植株遭受的逆境胁迫较小,强化了高温管理大棚嫁接黄瓜抵御逆境胁迫的能力,降低了逆境胁迫的程度,缓解了逆境伤害。

The Effect of Air Humidity on the Stress Physiology of the Grafted Cucumber in High Temperature Plastic Tunnel under CO2 Enrichment

The purpose was to study the effects of air humidity on stress physiology of the grafted cucumber in high temperature in plastic tunnel under CO2 enrichment.With the cucumber variety Jinchun 4 as the tested material,the cucumber plants were made for 3 treatments of air humidity as(95±5)%(L1),(75±5)%(L2),(55±5)%(L3)in high temperature of 40-45℃ under CO2 enrichment respectively.The result showed osmoregulation substances had an increased tendency with the treating days;MDA of the treatment L2 and L3 was increased and L1 was increased first and then decreased;cell membrane permeability of cucumber leaves of treatment L2 and L3 was increased and L1 was decreased;the cell protective enzymes activities of L1 and L2 was all increased and L3 was increased first and then decreased with the treating day.It was revealed that the plants had self-adjust abilities of stress tolerance.Proline content,MDA,the cell protective enzymes activities and cell membrane permeability of cucumber leaves had a decreased tendency respectively with the treating days and showed L1﹤L2﹤L3.The soluble protein of treatment L1 was decreased 17.23% and 18.61% respectively compared to L2 and L3 after treatment 24 d.The same situation was for proline content was decreased 51.28% and 70.72%;soluble sugar was decreased 24.02% and 37.21%;MDA was decreased 43.44% and 55.28%;cell membrane permeability of cucumber leaves was decreased 74.46% and 74.46%;SOD activity was decreased 41.88% and 38.19%;CAT activity was decreased 61.21% and 67.35%;POD activity was decreased 27.80% and 39.45%.These results illustrated that the grafted cucumber plants was protected from stress tolerance in higher air humidity(95±5)% with high temperature plastic tunnel under CO2 enrichment.The ability of resistance stress tolerance of grafted cucumber plants was increased and hurt of plants was alleviated by stress tolerance under higher air humidity with high temperature under CO2 enrichment.