利用种子性状QTL定位高油玉米淀粉含量QTL

以普通玉米自交系8984与高油玉米自交系GY220为亲本组配得到284个F2:3家系群体,利用185个SSR标记构建玉米遗传连锁图谱。通过包含母体效应的种子性状QTL作图方法对玉米籽粒淀粉含量进行QTL定位和效应分析,共检测到7个与淀粉含量相关的QTL,分别位于第5,8,10染色体上,除qSTA8-3的遗传作用方式表现为加性外,其余QTL作用方式为部分显性。单个QTL贡献率为5.87%～10.93%,累计贡献率为53.37%。所有QTL的增效基因均来自普通玉米亲本8984。淀粉含量QTL qSTA5-2贡献率较大,可以作为进一步精细定位的主要目标QTL。

QTL Analysis of Starch Content in High-oil Maize Using Seed Trait QTL

F2:3 populations consisted of 284 plants were developed from the cross between a normal maize inbred 8984 and a high-oil maize inbred GY220,and a genetic linkage map including 185 SSR markers constructed by(8984/GY220) F2 population.The quantitative trait loci(QTL) of starch content were identified and their effects were evaluated using SeedTraitQTL containing maternal effect.A total of seven QTL were detected in the F2:3 population,located on chromosome 5,8 and 10 respectively.Six QTLs showed partially dominant effects but qSTA8-3 showed additive.Contribution of single QTL to phenotypic variation varied from 5.87% to 10.93%,and total contributions of all QTLs were 53.38%.The allele of all QTLs were contributed by the normal maize parent 8984.The QTL qSTA5-2 with higher contribution could be used as the main objective QTL in fine mapping.