一株Agaricus属野生菌的分离与鉴定

为了对一株大型野生真菌进行分离及鉴定,通过组织分离获得该野生菌的菌丝纯培养物,分别提取子实体及菌丝体的基因组DNA,并以此为模板进行ITS片段的扩增、测序及系统发育分析。结果表明,从菌丝体和子实体中菌扩增获得了750 bp左右的ITS片段,经序列测定表明,菌丝纯培养物来源于该子实体,比对结果表明,该真菌属于Agaricus属,GenBank登录号为JN907015。系统发育分析表明了该菌在属内的遗传地位。建立了野生大型真菌的鉴定方法,为该野生资源的利用奠定了基础。

Isolation and Identification for a Wild Fungus of Genus Agaricus

The aim of this study is to identify an isolated fungus from wild environment.This study established a method for the identification of the wild fruiting body forming fungi.Method:The mycelium was isolated from the fruiting body by using PDA medium.Genomic DNA was extracted from not only mycelium and fruiting body then used as template for the ITS gene PCR amplification.Further,phylogenetic analysis based on the ITS sequence was conducted to confirm the genetic position of this fungi.Results:About 750 bp fragment was amplified and sequenced.The results showed that ITS sequence from mycelium and fruiting body was uniform.Phylogenetic analysis indicated that the fungi belonged to the genus Agaricus.GenBank accession number is JN907015.Conclusion:The results established an identification method for the wild fruiting body forming fungus and laid the foundation of the application of this fungus.