| 应用巢式PCR检测鸭圆环病毒浙江株及其全基因序列分析 |
| |
| 引用本文： | 万春和,施少华,程龙飞,傅光华,陈红梅,黄瑜. 应用巢式PCR检测鸭圆环病毒浙江株及其全基因序列分析[J]. 福建农业学报, 2009, 24(5): 390-395 |
| |
| 作者姓名： | 万春和 施少华 程龙飞 傅光华 陈红梅 黄瑜 |
| |
| 作者单位： | 福建省农业科学院畜牧兽医研究所,福建,福州,350013;福建省畜禽疫病防治工程技术研究中心,福建,福州,350013 |
| |
| 基金项目： | 现代农业产业技术体系建设专项资金，福建省财政专项-福建省农科院科技创新团队建设基金，福建省农科院青年人才创新基金 |
| |
| 摘 要： | 应用巢式PCR（nested PCR）技术对收集自浙江温州种番鸭进行鸭圆环病毒核酸扩增，并对测定的序列进行分析，结果用套式引物扩增出目的条带片段大小约为340nt。根据序列测定结果设计1对反向引物用于测定病毒基因组余下的基因片段。序列分析表明该圆环病毒基因组全长为1995nt，其ORF-V1编码292个氨基酸，ORF—C1编码257个氨基酸。序列分析表明，本试验株与番鸭分离株（GenBank登录号EF451157）和半番鸭分离株（GenBank登录号EU344804）的同源性最高，均高达99．2％，与鸭圆环病毒欧洲（德国）分离株和北美（美国）分离株在同一支遗传进化关系上。
|
| 关 键 词： | 鸭圆环病毒 巢式PCR 序列分析 |
Nested PCR detection and sequence analysis on Zhejiang duck circovirus |
| |
| WAN Chun-he,SHI Shao-hua,CHENG Long-fei,FU Guang-hua,CHEN Hong-mei,HUANG Yu. Nested PCR detection and sequence analysis on Zhejiang duck circovirus[J]. Fujian Journal of Agricultural Sciences, 2009, 24(5): 390-395 |
| |
| Authors: | WAN Chun-he SHI Shao-hua CHENG Long-fei FU Guang-hua CHEN Hong-mei HUANG Yu |
| |
| Affiliation: | WAN Chun-he~(1,2),SHI Shao-hua~(1,CHENG Long-fei~(1,FU Guang-hua~(1,CHEN Hong-mei~(1,HUANG Yu~(1,2) (1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China,2.Fujian Animal Diseases Control Technology Development Center,China) |
| |
| Abstract: | Using the nested PCR technology,the duck circovirus(DuCV) nucleotide fragment,which was isolated from Wenzhou,Zhejiang,was amplified and its sequence analyzed.The result showed that the obtained segment was about 340nt in length.Based on the determined sequence,two primers were applied for the construction of the remaining genomic sequence by inverse PCR.The genome of the Zhejiang DuCV had a length of 1995nt.Its ORFV1 encoded a replication protein of 292 amino acids,while the ORF-C1 encoded a capsid protein... |
| |
| Keywords: | duck circovirus nested PCR sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
