番茄叶片蜡质和角质层与芝麻斑病菌侵染的关系

对4个抗芝麻斑病和6个感病番茄品种的叶片表皮抗性机制进行了研究。结果表明,采用氯仿去除叶片蜡质,用KOH或角质酶去除叶片角质层后,叶片芝麻斑病发病率显著增加;叶片蜡质和角质层同时去除的与叶片破损的芝麻斑病发病率差异不显著,表明叶片的抗性成分主要是蜡质和角质层。叶片蜡质含量测定结果表明,蜡质含量和角质层的厚度与品种的抗性有关。抗病品种叶片蜡质含量显著高于感病品种。叶片蜡质提取物在体外抑菌效果不显著,说明蜡质的抗性作用主要是物理性阻止芝麻斑病菌的穿透。

Relationship between Tomato Leaf Wax and Cutin Layers with Infection by Helminthosporium carposaprum

When the Wax in tomato leaves was removed with chloroform or the cutin layer was removed with KOH or cutinase，fungal infection rate in tomato leaves，was significantly increased.There was no significant difference in Helminthosporium carposaprum infection rate between leaves whose wax and Cutin within were removed at the same time and leaves damaged by pin puncturing，indicating that the major anti-disease components in leaves were wax and cutin.Wax extract in leaves antibacterial effect was not significant in vitro.Wax resistance role is mainly to prevent physical penetration Helminthosporium carposaprum.