| 蛹虫草液体菌种培养基的优化 |
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| 引用本文： | 徐方旭,刘诗扬,王升厚. 蛹虫草液体菌种培养基的优化[J]. 北方园艺, 2011, 0(4): 199-201 |
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| 作者姓名： | 徐方旭 刘诗扬 王升厚 |
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| 作者单位： | 沈阳师范大学特种菌业研究所,辽宁沈阳,110034 |
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| 基金项目： | 辽宁省自然科学基金资助项目 |
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| 摘 要： | 通过正交实验优化蛹虫草液体菌种培养基,以干菌体得率为指标,选择出蛹虫草液体发酵的最适培养基。结果表明:最适液体培养基配方为:葡萄糖30 g/L,蛋白胨16 g/L,磷酸二氢钾6 g/L,硫酸镁2 g/L,水1 000 mL,pH自然,干菌体得率为46.5 g/L,与其它试验组结果的差异显著,说明碳源和氮源为其生长的最重要营养因素。
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| 关 键 词： | 蛹虫草 干菌体 液体培养基 因素 水平 |
Liquid Medium for Optimization of Cordyceps militaris |
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| XU Fang-xu,LIU Shi-yang,WANG Sheng-hou. Liquid Medium for Optimization of Cordyceps militaris[J]. Northern Horticulture, 2011, 0(4): 199-201 |
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| Authors: | XU Fang-xu LIU Shi-yang WANG Sheng-hou |
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| Affiliation: | XU Fang-xu,LIU Shi-yang,WANG Sheng-hou (Institute of Special Edible Fungi,Shenyang Normal University,Shenyang,Liaoning 110034) |
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| Abstract: | To optimize the liquid culture medium of Cordyceps militaris by orthogonal layout and with the desiccant weights of the mycelium being a index to choose the most suitable liquid medium for Cordyceps militaris.The results showed that the optimum liquid culture medium was glucose 30 g/L,peptone 16 g/L,KH2PO4 6 g/L,MgSO4 2 g/L, water 1 000 mL,pH natural.The desiccant weights of the mycelium was 46.5 g/L,with the other experimental groups significantly different results.The carbon and nitrogen conpound were the most important tropho-factor for the growth of Cordyceps militaris. |
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| Keywords: | Cordyceps militaris desiccant mycelium liquid medium factor level |
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