| G+Cmol%含量测定中细菌破壁方法的筛选与应用 |
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| 引用本文： | 李琳,李槿年,余为一.G+Cmol%含量测定中细菌破壁方法的筛选与应用[J].安徽农业科学,2005,33(2):283-285. |
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| 作者姓名： | 李琳 李槿年 余为一 |
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| 作者单位： | 安徽农业大学畜牧水产学院,安徽合肥,230036;安徽农业大学畜牧水产学院,安徽合肥,230036;安徽农业大学畜牧水产学院,安徽合肥,230036 |
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| 基金项目： | 安徽省自然科学基金
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安徽省高等学校优秀人才计划 |
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| 摘 要： | 以标准株大肠杆菌K1 2 为材料对 3种细菌破壁方法 (SU法、ES法和ESU法 )进行了比较 ,并在此基础上测定了 17株病原菌的G +Cmol%含量。结果表明 :ESU法破壁提取的DNA含量最大 ,纯度最高 ,热变性曲线幅度最大 ,G +Cmol%含量符合文献值 ,17株病原菌鉴定至种 (11/ 17)或属 (6/ 11) ;在 3种破壁方法中 ,ESU法最有效 ,适用于水产动物病原菌的种属鉴定。
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| 关 键 词： | 细菌 破壁方法 Tm法 DNA G+Cmol |
| 文章编号： | 0517-6611(2005)02-0283-03 |
Choose of Optimal Method of Breaking Bacterial Cellular Wall in G+Cmol% Dermination and Identification of 17 Strains of Bacteria |
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| LI Lin et al.Choose of Optimal Method of Breaking Bacterial Cellular Wall in G+Cmol% Dermination and Identification of 17 Strains of Bacteria[J].Journal of Anhui Agricultural Sciences,2005,33(2):283-285. |
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| Authors: | LI Lin |
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| Abstract: | Escherichia coli K_ 12 was used to pick out optimal method of breaking bacterial cellular wall,DNA qualities and G+Cmol% were compared among the three experimental ways,namely ES(lysozyme plus SDS), SU(SDS plus ultrasonic), ESU(lysozyme plus SDS and ultrasonic). ESU is the best method Judged by DNA purity and content,denaturation curve and G+Cmol%, 17 strains of bacteria were identified to species(11/17) or genus(6/17). Optimal method of breaking bacterial cellular wall was offered for G+Cmol% determination exactly and used for identification of aquatic pathogenic bacteria. |
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| Keywords: | Bacteria Breaking method Tm DNA G+Cmol% |
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