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| 禽流感病毒H5亚型金标快速诊断技术方法的建立及试剂盒研制 | |
| 引用本文： | 李克生,杜惠芬,连晓雯,徐海聂,薄清如,黄新民,才学鹏. 禽流感病毒H5亚型金标快速诊断技术方法的建立及试剂盒研制[J]. 农业生物技术学报, 2010, 18(2): 400-405. DOI: 10.3969/j.issn.1674-7968.2010.02.032 |
| 作者姓名： | 李克生 杜惠芬 连晓雯 徐海聂 薄清如 黄新民 才学鹏 |
| 作者单位： | 1. 甘肃农业大学,兰州,730070;甘肃省医学科学研究院,兰州,730030 2. 甘肃省医学科学研究院,兰州,730030;兰州雅华生物技术有限公司,甘肃省生物检测工程技术研究中心,兰州,730030 3. 珠海出入境检验检疫局,珠海,519015 4. 中国农业科学院兰州兽医研究所,兰州,730046 |
| 基金项目： | 科技部农业科技成果转化资金项目 |
| 摘 要： | 应用纯化禽流感病毒(AIV)H5抗原免疫Balb/c小鼠,得到2株配对良好的抗AIV H5单抗;采用金标免疫层析法制备AIV H5亚型金标快速诊断试剂盒,并对试剂盒性能进行评价.实验结果表明,AIV-H5金标试剂盒与AIV H5以外其它亚型及其它禽类病毒不发生交叉反应,其灵敏度达1/27,稳定性良好:试剂盒检测人工感染AIV H5的鸡脏器组织,结果除心脏、泄殖腔拭子和喉拭子外,其余脏器均呈阳性,与鸡胚病毒分离培养结果相符;田间试验结果与荧光RT-PCR试剂盒检测符合率达100%. |
| 关 键 词： | 禽流感病毒 快速诊断技术 试剂盒 |
Development of Rapid Detecting Method and Lateral Flow Assay of H5 Subtype Avian Influenza Virus |
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| Li Kesheng,Du Huifen,Lian Xiaowen,Xu Hainie,Bo Qingru,Huang Xinmin,Cai Xuepeng. Development of Rapid Detecting Method and Lateral Flow Assay of H5 Subtype Avian Influenza Virus[J]. Journal of Agricultural Biotechnology, 2010, 18(2): 400-405. DOI: 10.3969/j.issn.1674-7968.2010.02.032 | |
| Authors: | Li Kesheng Du Huifen Lian Xiaowen Xu Hainie Bo Qingru Huang Xinmin Cai Xuepeng |
| Affiliation: | Li Kesheng 1,2 Du Huifen 2,3 Lian Xiaowen 2,3 Xu Hainie 4 Bo Qingru 4 Huang Xinmin 4 Cai Xuepeng 5 1 Gansu Agriculture University,Lanzhou,730070,2 Gansu Institute of Medical Secicne,730030,3 Lanzhou Yahua Biotech Co.Ltd,Gansu Province Biotech , Engineeering Research Center,4 Zhuhai Entry-Exit Inspection , Quarantine Bureau,Zhuhai,519015,5 Lanzhou Veterinary Institute,Chinese Sciences Academy of Agriculture,730046 |
| Abstract: | Balb/c mice were immunized by purified Avian influenza virus(AIV) H5 antigen, 2 cells of monoclonal antibody(McAb) which anti-AIV-H5 had good antigen matching, were produced.One kind of AIV-H5 lateral flow assay was developed by using gold immunochromatography, and its capability were evaluated.Experiment results showed that the AIV-H5 lateral flow assay had no cross-reaction with other substype of AIV and other fowlery viruses , and had high sensibility(1/27) and good stability.The man-made infected chicke... |
| Keywords: | Avian influenza virus Rapid diagnostic techniques Lateral flow assay |
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