| 不同保藏方法对香菇菌种酶活性影响 |
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| 引用本文： | 刘新锐,宋秀高,邱昌颖,王玉青,谢宝贵.不同保藏方法对香菇菌种酶活性影响[J].江西农业大学学报,2012(1):170-174. |
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| 作者姓名： | 刘新锐 宋秀高 邱昌颖 王玉青 谢宝贵 |
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| 作者单位： | 福建农林大学菌物研究中心;福建省农业区划研究所 |
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| 基金项目： | 公益类科研院所专项项目(2009R10009-1) |
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| 摘 要： | 为研究香菇菌种在不同保藏方法后的酶活性变化,用液氮保藏、4℃保藏、矿物油保藏、25℃保藏等4种保藏方法对香菇菌种进行保藏试验,同时测定保藏后的生长速度和酶活性。研究结果可知:液氮保藏对维持香菇菌种活力的效果最好,香菇菌种经液氮保藏后的生长速度最快,并且其漆酶、羧甲基纤维素酶(CMC酶)、木聚糖酶和淀粉酶的活性最高,而矿物油保藏的生长速度最慢和酶活性最低。结论:上述4种酶活力可作为香菇菌种保藏效果的评价指标。
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| 关 键 词： | 香菇菌种 生长速度 酶活力 菌种保藏 |
Effect of Different Preservation Methods on the Enzyme Activity of Lentinula edodes Spawn |
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| LIU Xin-rui,SONG Xiu-gao,QIU Chang-ying,WANG Yu-qing,XIE Bao-gui.Effect of Different Preservation Methods on the Enzyme Activity of Lentinula edodes Spawn[J].Acta Agriculturae Universitis Jiangxiensis,2012(1):170-174. |
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| Authors: | LIU Xin-rui SONG Xiu-gao QIU Chang-ying WANG Yu-qing XIE Bao-gui |
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| Institution: | 1*(1.Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2.Agricultural Program Institute of Fujian Province,Fuzhou 350003,China) |
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| Abstract: | In order to study the enzyme activation change of Lentinula edodes spawn after preservation,liquid nitrogen preservation,4 ℃ preservation,paraffin preservation and 25 ℃ preservation were used to preserve Lentinula edodes.Growth rate and enzyme activity were detected after preservation.The results showed that liquid nitrogen preservation was the best preservation method.The growth rate was the highest,and the enzyme activities(laccase,CMC,xylanase,alpha amylase) were also the highest after liquid nitrogen preservation,while paraffin preservation was the opposite.It was indecated that laccase,CMC,xylanase and alpha amylase activity could be regarded as evaluating indexes of effects of preservation methods for the preservation of Lentinula edodes spawn. |
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| Keywords: | Lentinula edodes spawn growth rate enzyme activation spawn preservation |
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