Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing |
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Authors: | Berrilli Federica Di Cave David De Liberato Claudio Franco Alessia Scaramozzino Paola Orecchia Paola |
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Affiliation: | Cattedra di Parassitologia, Dipartimento di Sanità Pubblica e Biologia Cellulare, Università di Roma Tor Vergata, Via Montpellier 1, 00133 Rome, Italy. berrilli@uniroma2.it |
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Abstract: | In order to investigate the genotypes of Giardia duodenalis from domestic and farm animals in Italy, 21 Giardia isolates, 17 from dogs, 1 from cat and 3 from dairy calves, were genetically characterised by SSU-rRNA gene sequencing. Among dogs, 76.5% of isolates showed the dog-specific genotypes (Assemblages C, D and C/D mixed Assemblage) and 23.5% exhibit potential zoonotic genotypes (Assemblage A and A/C mixed Assemblages). The cat isolate belonged to assemblage A, whereas the sequences among the isolates from calves were found to correspond to hoofed-livestock genotype, namely Assemblage E. These findings suggest that infection of humans by zoonotic genotypes from domestic animals could be of low epidemiological significance, although possible. The present study represents the first contribute to the knowledge of G. duodenalis genotypes in domestic and farm animals from Italy. |
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