Micropropagation of Bolaina blanca (Guazuma crinita Mart.), a fast-growing tree in the Amazon region |
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| Authors: | Emilio Maruyama Katsuaki Ishii Isao Kinoshita Kihachiro Ohba Akira Saito |
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| Affiliation: | (1) Institute of Agriculture and Forestry, University of Tsukuba, 305 Tsukuba, Japan;(2) Forestry and Forest Products Research Institute, 305 Ibaraki, Japan;(3) Faculty of Agriculture, Kyushu University, 812 Fukuoka, Japan |
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| Abstract: | Rapid clonal propagation of Bolaina blanca (Guazuma crinita Mart.) was established by the subculturing of the shoot-tips from aseptically germinated seedlings on woody plant medium (WPM) supplemented with trans-zeatin [trans-6-(4-Hydroxy-3-methylbut-2-enylamino)purine] (ZEA). After 45 days of culture, a seven-fold multiplication rate was achieved on WPM containing 10 μM of ZEA. Obtained shoots were simultaneously elongated and rooted on WPM containing 1 μM of kinetin [6-furfurylaminopurine] (KIN). After 60 days of culturing the growth of shoots was evident, and high rooting percentages were obtained. The plantlets were transferred into pots with vermiculite and acclimatized successfully in plastic boxes with transparent cover inside the growth cabinet. |
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| Keywords: | bolaina blanca fast-growing tree Guazuma crinita micropropagation tropical tree |
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