桃幼胚离体培养再生植株的研究

 以中晚熟桃品种‘京艳’、‘绿化9 号’、‘ 晚蜜’和‘大久保’为试材, 初步建立了桃体细胞胚发生和增殖的三阶段程序, 即胚性愈伤组织诱导、体细胞胚发生和发育、体胚萌发成株以及次级体细胞胚的增殖。各品种体细胞胚的萌发成株率分别为73. 5 %、38. 6 %、5. 4 %和58. 2 %。用苯脲类细胞分裂素TDZ有效诱导‘京艳’的幼胚子叶直接再生植株, 再生率70 % , 平均每外植体不定芽数13. 8 个, 再生不定芽生根后获得完整植株。

Plant Regeneration from Excised Immaturate Embryos of Peach (Prunus persica L.)

A three-stage culture program of somatic embryogenesis , consist of induction of embryogenic callus , formation and development of somatic embryos , regeneration of intact plantlets , was developed in in four peach (Prunus persica) cultivars , ‘Jingyan’, ‘Lühua 9’, ‘Wanmi’and ‘Dajiubao’. The percentage of germination and plant regeneration from normally developed soamtic embryos in the cultivars was 73. 5 % , 38. 6 % , 5. 4 % and 58. 2 % , respectively. TDZ is effective in stimulating adventitious bud regeneration from immature cotyledons of ‘Jingyan’, 70 % differentiation rate and 13. 8 adventitious buds per cotyledon were obtained. The regenerated adventitious shoots rooted at a rate of 90 %.