素花党参多糖的超声提取和含量测定 |
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引用本文: | 何先元,陈媛媛,许晋芳,王秋霜.素花党参多糖的超声提取和含量测定[J].农技服务,2009,26(11):135-136. |
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作者姓名: | 何先元 陈媛媛 许晋芳 王秋霜 |
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作者单位: | 重庆医科大学中医药学院,重庆,401331 |
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摘 要: | 目的]从素花党参中提取多糖并测定其含量方法]用超声提取素花党参多糖;用苯酚—硫酸法测定其含量。结果]测得素花党参中多糖含量为30.49%,平均收回率为99.24%,RSD=2.32%(n=6)。结论]甘肃素花党参中多糖含量高,具有开发利用价值。超声提取是一种有效的多糖提取方法。
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关 键 词: | 党参 多糖 超声提取 苯酚—硫酸法 |
Ultrasonic Extraction and Determination of Polysaccharide in Radix Codonopsis |
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Institution: | HE Xian-yuan et al (College of Traditional Chinese Medical,Chongqing Medical University,Chongqing401331 ) |
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Abstract: | Objective ] To extract the polysaceharide and measure the polysaeeharide content in Radix Codonopsis pilosulae. Method ] The polysaecharide was obtained with the water extraction and alcohol precipitation method, and the polysaecharide content of the extraction was measured with the Phenol-sulfuric acid method. Result ] The polysaecharide content in Radix Codonopsis pilosulae was 30.49% , the rate of recovery was 99.24%, and RSD was 2.32% ( n = 6 ). Conclution ] Polysaccharide content of Radix Codonopsis pilosulae in Gansu was high, and it has higher value of development and utilization. Ultrasonic extraction was an effective method of polysaccharide extract. |
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Keywords: | Radix Codonopsis pilosulae Polysaccharide Ultrasonic extractic Phenol - sulfate acid method |
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