| 利用CRISPR/Cas9创建osarf7突变体及其农艺性状调查 |
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| 引用本文： | 李兆伟,孙聪颖,零东兰,曾慧玲,张晓妹,范凯,林文雄. 利用CRISPR/Cas9创建osarf7突变体及其农艺性状调查[J]. 中国水稻科学, 2022, 36(3): 237-247. DOI: 10.16819/j.1001-7216.2022.210117 |
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| 作者姓名： | 李兆伟 孙聪颖 零东兰 曾慧玲 张晓妹 范凯 林文雄 |
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| 作者单位： | 1.福建农林大学 生命科学学院/福建省农业生态过程与安全监控重点实验室, 福州 350002;2.福建农林大学 农学院/教育部作物遗传育种与综合利用重点实验室, 福州 350002 |
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| 基金项目： | 国家自然科学基金资助项目(31701329);福建省自然科学基金资助项目(2021J01098);中国博士后科学基金资助项目(2015M580560) |
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| 摘 要： | [目的]探究OsARF7基因在水稻生长发育中的生物学功能及其对水稻农艺性状的影响.[方法]利用CRISPR/Cas9技术,在粳稻中花11背景下对OsARF7进行定向编辑,获得OsARF7基因突变植株,并考查其农艺性状.[结果]获得22株T0代转基因株系,经PCR扩增潮霉素基因鉴定出20株阳性植株.提取T2代转基因植株的...
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| 关 键 词： | 水稻 基因编辑 OsARF7 CRISPR/Cas9 生长素 |
| 收稿时间： | 2021-01-17 |
| 修稿时间： | 2021-04-22 |
Construction of osarf7 Mutants in Rice Based on CRISPR/Cas9 Technology and Investigation on Their Agronomic Traits |
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| LI Zhaowei,SUN Congying,LING Donglan,ZENG Huiling,ZHANG Xiaomei,FAN Kai,LIN Wenxiong. Construction of osarf7 Mutants in Rice Based on CRISPR/Cas9 Technology and Investigation on Their Agronomic Traits[J]. Chinese Journal of Rice Science, 2022, 36(3): 237-247. DOI: 10.16819/j.1001-7216.2022.210117 |
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| Authors: | LI Zhaowei SUN Congying LING Donglan ZENG Huiling ZHANG Xiaomei FAN Kai LIN Wenxiong |
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| Affiliation: | 1.College of Life Sciences, Fujian Agriculture and Forestry University / Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fuzhou 350002, China;2.College of Agriculture, Fujian Agriculture and Forestry University / Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fuzhou 350002, China |
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| Abstract: | 【Objective】The purpose is to offer a deep insight into the biological function of OsARF7 and its effect on agronomic traits during the growth and development stage of rice. 【Method】CRISPR/Cas9 technology was employed to edit the gene sequence of OsARF7 and to obtain the mutants of japonica variety Zhonghua 11. The agronomic traits of the mutants were investigated in a field experiment. 【Result】Twenty-two T0 transgenic lines were obtained through Agrobacterium-mediated infection of japonica rice Zhonghua 11 callus, and twenty positive transgenic lines were distinguished by screening the gene encoding hygromycin. For T2 transgenic lines, the regions of genomic DNA including two target sites were detected and identified by PCR amplifying and sequencing, and fifteen homozygous mutations were confirmed. Gene expression results displayed that the expression levels of OsARF7, OsPIN1b, OsPIN2, and OsLAX2 in the osarf7 mutants were significantly lower than those in the wild type. In comparison with the wild-type plants, osarf7 mutants showed a significant decrease in the productive panicle percentage. Among them, the osarf7 mutants with more tillers than the wild type showed a similar effective panicle number to the wild type, and the osarf7 mutants with the same tiller number as the wild type presented a decrease in the effective panicle number per plant in paddy field conditions, resulting in increased non-productive tillers and decreased effective panicle percentage. 【Conclusion】OsARF7 was involved in the emergence and development of tiller bud dominated by auxin, and the successful construction of osarf7 mutants plays a positive role in revealing the biological function of OsARF7 and the mechanism of tiller formation and development dominated by auxin. |
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| Keywords: | rice gene editing OsARF7 CRISPR/Cas9 auxin |
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