Appearance of slow-reacting and complement-requiring neutralizing antibody in cattle infected with Akabane virus |
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Authors: | K Sato Y Tanaka S Tokuhisa Y Inaba T Fushiki M Matumoto |
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Institution: | National Institute of Animal Health, Ibaraki, Japan. |
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Abstract: | Slow-reacting complement-requiring neutralizing (NT) antibody was detected in sera from cattle 2 weeks after infection with Akabane virus. Bovine sera obtained 3 or 4 weeks after infection contained slow-reacting noncomplement-requiring NT antibody. The slow-reacting complement-requiring NT antibody was sensitive to 2-mercaptoethanol (2-ME), whereas the slow-reacting noncomplement-requiring NT antibody was resistant to 2-ME. The initial phase may represent the IgM response and the later phase a change to IgG. A NT test was developed in which virus-serum mixtures were incubated at 4 degrees C for 48 h and then with complement at 37 degrees C for 60 min; this gave an improved sensitivity over the previous incubation at 37 degrees C for 60 min. |
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