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| 枯草芽孢杆菌离子突变菌株H-74脂肽类抗菌物质对水稻抗性诱导作用 | |
| 引用本文： | 张荣胜,刘永锋,刘邮洲,罗楚平,刘长河,李美荣,陈志谊.枯草芽孢杆菌离子突变菌株H-74脂肽类抗菌物质对水稻抗性诱导作用[J].西南农业学报,2009,22(5). |
| 作者姓名： | 张荣胜 刘永锋 刘邮洲 罗楚平 刘长河 李美荣 陈志谊 |
| 作者单位： | 1. 江苏省农业科学院植物保护研究所,江苏,南京,210014;南京农业大学植物保护学院农业部作物病虫害监测与防控重点开放实验室,江苏,南京,210095 2. 江苏省农业科学院植物保护研究所,江苏,南京,210014 3. 南京农业大学植物保护学院农业部作物病虫害监测与防控重点开放实验室,江苏,南京,210095 |
| 基金项目： | 国家"863"计划现代农业技术领域重大项目，江苏省自然科学基金，江苏省农科院基金 |
| 摘 要： | 通过离子注入技术对枯草芽孢杆菌生防菌Bs-916突变选育,得到一株拮抗性能比出发菌Bs-916提高15 %以上的突变菌株H-74.通过对其抗菌物质的高效液相色谱(HPLC)分析表明,H-74分泌的脂肽类抗菌物质主要是表面活性素.在PDA平板上的水稻纹枯病菌菌丝抑制试验中,它能有效抑制新生菌丝的生长,并使细胞内含物外泄.盆栽控病试验表明,它对水稻纹枯病菌的相对防效达到73.35 %.将H-74分泌的脂肽类抗菌物质单喷于水稻植株上,测定与植物抗病相关的超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)活性的变化,发现其对植物抗病相关酶活性表达有明显的诱导作用.结果还显示同时接种脂肽类抗菌物质和水稻纹枯病菌处理对3个酶活性影响最大. |
| 关 键 词： | 枯草芽胞杆菌 表面活性素 酶活性 诱导抗性 |
Effect of Antibacterial Lipopeptide from Bacillus subtilis H-74 on Resistance Induction of Rice |
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| ZHANG Rong-sheng,LIU Yong-feng,LIU You-zhou,LUO Chu-ping,LIU Chang-he,LI Mei-rong,CHEN Zhi-yi.Effect of Antibacterial Lipopeptide from Bacillus subtilis H-74 on Resistance Induction of Rice[J].Southwest China Journal of Agricultural Sciences,2009,22(5). | |
| Authors: | ZHANG Rong-sheng LIU Yong-feng LIU You-zhou LUO Chu-ping LIU Chang-he LI Mei-rong CHEN Zhi-yi |
| Abstract: | A mutant strain H-74 of which antagonistic performance was increased by 15 % than that of Bs-916 was obtained by the mutation selection to biocontrol bacteria Bs-916 by ion implantation technique ionexclusion . High Performance Liquid Chromatography (HPLC) analysis indicated the antibacterial lipopeptide from Bacillus subtilis H-74 was mainly surfactin.The antibiosis detection to Rhizoctonia solani in plate showed antibacterial lipopeptide from Bacillus subtilis H-74 could effectively inhibit mycelial growth of Rhizoctonia solani and make cell inclusion released. In the pots experiments, the control efficiency to Rhizoctonia solani was 73.35 %.The results showed that antibacterial lipopeptide from Bacillus subtilis had induced effects on the activity of SOD, POD and PPO. Moreover,the results showed that when Rhizoctonia solani with single-jet lipopeptides was inoculated,the activity of SOD, POD and PPO was the highest |
| Keywords: | Bacillus subtilis Surfactin Enzyme activity Resistance induction |
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