Ribosomal RNA phylogeny of bacterial and fungal pathogens of Agriotes wireworms |
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Authors: | Andreas Leclerque Polina V Mitkovets Ana-Cristina Fatu Christina Schuster Regina G Kleespies |
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Institution: | 1. Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Biological Control, Heinrichstra?e 243, 64287, Darmstadt, Germany 2. Technische Universit?t Darmstadt, Institut für Mikrobiologie und Genetik, Schnittspahnstra?e 10, 64287, Darmstadt, Germany 3. All-Russian Institute for Plant Protection, Chaussee Podbel’skogo 3, 196608, St. Petersburg, Russia 4. Research-Development Institute for Plant Protection (ICDPP), Blvd. Ion Ionescu de la Brad 8, 013813, OP 18, Bucharest, Romania 5. Julius Kühn-Institut (JKI), Institut für Biologischen Pflanzenschutz, Heinrichstra?e 243, 64287, Darmstadt, Germany
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Abstract: | Wireworms, the polyphagous larvae of click beetles belonging to the genus Agriotes (Coleoptera: Elateridae), are severe and widespread agricultural pests affecting numerous crops. Biological control agents and methods for this general pest are highly solicited. In a screening for microbial Agriotes pathogens, an intracellular bacterium and a mitosporic fungus were isolated. Phylogenetic analysis based on ribosomal RNA operon sequences of both micro-organisms corroborated their previous morphology-based taxonomic classification. The bacterial pathogen has been assigned to the taxonomic genus Rickettsiella (Gammaproteobacteria) wherein it represents a new pathotype, ‘Rickettsiella agriotidis’, that appears most closely related to subjective synonyms of the nomenclatural type species, Rickettsiella popillae. The fungal pathogen has been shown to belong to the form-species Beauveria bassiana, i.e., an obligate anamorph related to the genus Cordyceps (Ascomycota: Hypocreales). Furthermore, the B. bassiana strain from Agriotes has been shown to be potentially susceptible to identification by gIi-diagnosis, i.e., a diagnostic method making use of the strain-specific presence of self-splicing group-I introns within the ribosomal RNA operons of certain hyphomycetous fungi. |
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