Induction of dendritic cell-mediated immune responses against canine malignant melanoma cells |
| |
Authors: | Tamura Kyoichi Yamada Misato Isotani Mayu Arai Hiroyoshi Yagihara Hiroko Ono Kenichiro Washizu Tsukimi Bonkobara Makoto |
| |
Affiliation: | Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan. |
| |
Abstract: | To establish the basis for the use of dendritic cells (DC) in the treatment of canine melanoma, dogs were vaccinated using autologous DC pulsed with canine melanoma CMM2 cell lysate in the presence of keyhole limpet haemocyanin (KLH) in vitro (CMM2-KLH-DC), and the induction of immune responses against CMM2 cells in vivo was examined using the delayed-type hypersensitivity (DTH) skin test. The DTH responses against CMM2 cells and KLH were observed in dogs vaccinated with CMM2-KLH-DC, while the responses against KLH but not CMM2 cells were detected with DC pulsed with KLH alone (KLH-DC). Recruitment of CD8 and CD4 T cells was detected in the positively responding sites, suggested that vaccination with CMM2-KLH-DC efficiently elicits T cell-mediated immunity against CMM-2 cells in vivo. These findings demonstrate the potential utility of DC-based tumour vaccination in the treatment of canine malignant melanoma. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|