转反义GhADF1基因对陆地棉纤维品质的影响

 【目的】将反义的棉花GhADF1基因导入陆地棉基因组,进一步了解抑制GhADF1基因对棉花纤维品质的影响。【方法】构建了GhADF1基因的反义植物表达载体,通过农杆菌介导法转化陆地棉（Gossypium hirsutum L.）品种Coker312。【结果】通过PCR检测卡那霉素抗性选择和系统选育获得3个不同转基因事件纯合系（A5、A9、A20）,Southern blot显示：反义GhADF1基因已整合到受体基因组中,且能稳定遗传。转基因纯合系连续3代纤维品质分析显示,它们的纤维比强度和长度与对照相比都显著提高。【结论】转反义GhADF1基因可以提高棉花纤维比强度和长度,改善棉纤维品质。

Effects of Transforming Antisense GhADF1 Gene on the Fiber Quality in Upland Cotton(Gossypium hirsutum L.)

Objective] To detect the effects of suppressing actin-depolymerizing factor gene of G hirsutum L.(GhADF1),antisense GhADF1 gene was introduced into upland cotton.Method]The ORF of GhADF1 was inserted into plant expression vector pPZP111 in the anti-sense orientation.The recombinant plasmid was introduced into cotton Coker312 (G.hirsutum L.) by the method of agrobacterium-mediated transformation.Result]PCR and Southern blot showed that the antisense GhADF1 gene was integrated into the cotton genome and could be inherited stably.The fiber quality traits analysis indicated that the fiber length and strength of 3 pure transgenic lines were significantly improved compared with that of Coker312.Conclusion]Transformation of antisense GhADF1 gene could improve fiber strength and cotton fiber quality.