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| 根癌农杆菌介导的水稻两用不育系培矮64S遗传转化体系的建立 | |
| 引用本文： | 肖媛,李落叶,徐孟亮,崔延春,夏新界.根癌农杆菌介导的水稻两用不育系培矮64S遗传转化体系的建立[J].农业现代化研究,2009,30(3):364-368. |
| 作者姓名： | 肖媛 李落叶 徐孟亮 崔延春 夏新界 |
| 作者单位： | 1. 中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南,长沙,410125;中国科学院研究生院,北京,100049 2. 中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南,长沙,410125 3. 中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,湖南,长沙,410125;中国科学院研究生院,北京,100049;湖南师范大学生命科学院,湖南,长沙,410081 |
| 基金项目： | 中国科学院百人计划项目，中国科学院亚热带农业生态研究所青年人才领域前沿项目 |
| 摘 要： | 以水稻两用不育系培矮64S成熟胚为外植体,以不同组合培养基为诱导和继代培养基,建立了适合水稻培矮64S转化的高效再生体系,并通过农杆菌EHA105介导,将大肠杆菌C1基因转入培矮64S,获得再生植株.结果表明.J0N1D3为合适的愈伤组织诱导培养基,诱导率达56.44%;通过潮霉素筛选后,获得的抗性愈伤组织分化率为73.08%,经分化,获得133株再生植株;随机挑选67株再生植株经PER检测,其中46株检测到目的条带,阳性检出率占68.66%;对PCR阳性植株进行荧光定量实时PCR分析,结果表明C1基因能在转基因植株中表达.通过对T1代PCR分析,得到目的条带,表明C1基因能在转基因后代稳定遗传. |
| 关 键 词： | 水稻 培矮64S 成熟胚 根癌农杆菌 转化体系 组织培养 |
Establishment of Agrobacterium-Mediated Transformation System of Thermo-Sensitive Genic Male Sterile Rice Pei'ai 64S |
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| XIAOYuan,LILuo-Ye,XUMeng-liang,CUIYan-chun and XIAXin-jie.Establishment of Agrobacterium-Mediated Transformation System of Thermo-Sensitive Genic Male Sterile Rice Pei'ai 64S[J].Research of Agricultural Modernization,2009,30(3):364-368. | |
| Authors: | XIAOYuan LILuo-Ye XUMeng-liang CUIYan-chun and XIAXin-jie |
| Institution: | 1.Key Laboratory for Agro-ecological Processes in Subtropical Region;Institute of Subtropical Agriculture;Chinese cademy of Sciences;Changsha;Hunan 410125;China;2.Graduate University of Chinese Academy of Sciences;Beijing 100049;3.Life Science College;Hunan Normal University;Hunan 410081;China |
| Abstract: | A high efficient regeneration system of rice plants for genetic transformation was developed through optimizing culture media,when mature embryos of Pei'ai 64S were used as explants.C1 gene cloned from E.coil was successfully delivered into Pei'ai 64S through the Agrobacterium-mediated method,and stable transgenic plants were obtained.The rate of callus induction reached 56.44% using J0N1D3 as the induction medium.A total of 133 hygromycin resistant plants were obtained with a regeneration rate of 73.08%.A ... |
| Keywords: | rice Pei'ai 64S mature embryo Agrobacterium tumefaciens transformation system tissue culture |
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