马传染性贫血病马与弱毒疫苗免疫马的区别试验

 本文应用淋巴细胞杂交瘤技术研制出具有抗马传染性贫血病驴白细胞弱毒抗原株系特异性的单克隆抗体（McAb）的酶结合试剂，以斑点试验（DB）与琼脂免疫双扩散试验（ID）相结合的方法，用于马传贫病马与马传贫驴白细胞弱毒疫苗免疫马血清抗体的鉴别。使用本方法对339匹实验马进行测试，共检出马传贫病马11匹，马传贫驴白细胞弱毒疫苗免疫马239匹，既未接种弱毒疫苗又未感染马传贫马89匹。另外，对106匹人工马传贫病马和21匹马传贫弱毒疫苗免疫马作了病理学验证，结果与免疫学检测相符。疫区在清除病马之后，病情停息。疫苗免疫马经贸易成交，更换畜主后，再次检疫未发现马传贫病例。结果表明，本方法对马传贫病马与马传贫驴白细胞弱毒疫苗免疫马的区别诊断有实用价值。

DIFFERENTIAL TEST ON SERUM ANTIBODY FROM EIA HORSES WITH THAT FROM ATTENUATED EIA VACCINE IMMUNIZED HORSES

In present research, a diagnostic method, combining dot blot (DB)test with immunodiffusion(ID)test, was developed to differentiate serum antibody of equine infectious anemia (EIA) virus infected horses from that of EIA attenuated vaccine immunized ones. A monoclonal antibody (McAb) specific to EIA donkey leucocyte attenuated virus labeled with horseradish peroxidase (HRP) was used in DB test. Vaccinated horses appear positive in DB test while EIA horses are positive in ID test and healthy ones without vaccination are negative in both DB and ID tests. Total 339 experiment horses from various provinces and areas were examined by a combination of DB and ID test for serum antibodies to vaccine antigen or EIA virus. Of them, 11 horses were infected with EIA virus, 239 were immunized with the vaccine and 89 were healthy. The results above were comfirmed by the pathological examination in 106 EIA horses and 21 vaccinated ones. The EIA epidemic was under control in infected areas after positive horses were eliminated and new EIA cases have not been detected in the vaccinated horses even though repeated quarantines were carried on. It has been proved that the method established in the study is valuable for differentiation between EIA horses and vaccinated ones.