润州凤头白鸭不同组织矿物元素沉积及相关基因表达研究

为了探讨润州凤头白鸭不同组织中主要矿物元素的沉积规律及相关基因的mRNA表达水平,利用原子荧光光度计分别测定了0、21、35、49和63日龄润州凤头白鸭胸肌、腿肌、肝脏、背皮和胫骨组织中的镁、钾、铁、锌和硒等矿物元素含量,采用qRT-PCR法分析不同日龄肝脏组织中各矿物元素相关基因表达水平及其与矿物元素沉积之间的关系....

Mineral Element Depositions and Gene Expression Across Different Tissues of the Runzhou White Crested Ducks

In order to investigate the depositions of several mineral elements and the mRNA expression levels of mineral-related genes in different tissues of Runzhou white crested duck, the contents of Mg, K, Fe, Zn and Se in breast muscle, thigh muscle, liver, skin and tibia at 0, 21, 35, 49 and 63 days of age were measured by atomic fluorescence spectrophotometer. The mRNA expression levels of mineral-related genes were detected by qRT-PCR. The results revealed that the depositions of Mg and K were basically the same in all tissues, showing a significant positive correlation (P<0.05). In the breast muscle, thigh muscle and liver, the contents of almost all the mineral elements reached the peak value (P<0.05) at 49—63 days of age. The expression levels of Mg-related genes TRPM6 and TRPM7 were the highest at 21 and 49 days of age, respectively (P<0.05); the expression levels of K-related genes ATP1A1 and ATP1B1 were the highest at 49 days of age (P<0.05); the expression of Fe-related gene FTH1 significantly increased (P<0.05) with the change of age, which had the greatest expression at the period of 49—63 days of age (P<0.05); the expression levels of Zn-related genes ATP6 and ATP8 were the highest at 49 and 35 days of age, respectively (P<0.05); the expression levels of Se-related genes GPX1 and GPX4 were the highest at 63 days of age (P<0.05). In addition, there was a significant positive correlation between the expression level of TRPM7 and the deposition of Mg in liver (r=0.942, P<0.05). Mg and K could be used as joint indicators for the precise breeding of high quality strain of Runzhou white crested duck; 49—63 days of age could be used as the reference of the best marketing age; TRPM7 gene could be used as the key gene for the detection of Mg. The results of this study provided a theoretical basis for breeding index and breeding efficiency of small-sized high quality meat duck.