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茶树内生防病和农药降解菌的分离
引用本文:洪永聪,辛伟,来玉宾,翁昕,胡方平.茶树内生防病和农药降解菌的分离[J].茶叶科学,2005,25(3):183-188.
作者姓名:洪永聪  辛伟  来玉宾  翁昕  胡方平
作者单位:福建农林大学农药与化学生物学教育部重点实验室,福建,福州,350002;福建农林大学生命科学学院,福建,福州,350002
摘    要:对不同茶树品种的健叶和病叶上分离的内生细菌进行了筛选和鉴定。结果表明,茶树体内存在大量的内生细菌,各品种间内生细菌的数量为2.9×106~39.4×106cfu/(g?fw)。内生细菌的生物功能测定结果表明,菌株TL2的拮抗能力强,先接种菌株TL224h后再接种茶轮斑病菌的防病效果好;同时菌株TL2对氯氰菊酯也表现出较强的降解能力;另外菌株TL2能在茶树上内生定殖。经鉴定,菌株TL2为枯草芽孢杆菌(Bacillussubtilis)。

关 键 词:茶树  内生细菌  防病  农药降解  枯草芽孢杆菌
文章编号:1000-369X(2005)03-083-06
收稿时间:2005-01-10
修稿时间:2005-05-08

Isolation of Endophytic Antifungal and Pesticide Degrading Bacteria from Tea Plant
HONG Yong-cong,XIN Wei,LAI Yu-bin,WENG Xin,HU Fang-ping.Isolation of Endophytic Antifungal and Pesticide Degrading Bacteria from Tea Plant[J].Journal of Tea Science,2005,25(3):183-188.
Authors:HONG Yong-cong  XIN Wei  LAI Yu-bin  WENG Xin  HU Fang-ping
Abstract:The screening and identification of endophytic bacteria from the healthy and diseased leaves of various tea cultivar plants were conducted.Results showed that the endophytic bacteria were accounted in 2.9× 106~39.4×106 cfu/(g?fw) in tea cultivars plants.The TL2 stain showed strongly antagonistic activity against leaf fungal pathogens of tea-plant.It showed a good protecting effect when inoculated the TL2 strain bacteria firstly than inoculated the tea grey blight pathogen(Pestallozzia theae) later,Besides,it showed a strong degrative activity to cypermethrin.The TL2 bacteria strain could colonize within tea plant.According to the results of identification,the TL2 bacteria strain was identified as bacillas subtilis.
Keywords:Tea-plant  Endophytic Bacteria  Antifungal  Pesticide Degrading  Bacillus subtilis
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