Lactic acid bacteria effective for regulating the growth of contaminant bacteria during the fermentation of Undaria pinnatifida (Phaeophyta) |
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Authors: | Motoharu Uchida Masakazu Murata Fumiyasu Ishikawa |
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Affiliation: | National Research Institute of Fisheries and Environment of Inland Sea, Hatsukaichi, Hiroshima 739-0452,;National Research Institute of Fisheries Science, Yokohama, Kanagawa 236-8648, and;Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan |
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Abstract: | ABSTRACT: Lactic acid fermentation of seaweed is a recent topic and quite limited information is available on culture conditions. To know the suitable strains for use as a starter culture for seaweed fermentation, 14 lactic acid bacteria (LAB) strains, including 11 species, were tested in culture conditions prepared with or without salt. A commercial product of Undaria pinnatifida powder was used as a substrate for fermentation without sterilizing. Starter-suitability of the LAB strains was assessed from their predominance after culture. Among the tested strains, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosus showed high (>90%) predominance in their cultures, while control cultures prepared without inoculation of LAB did not show any detectable growth of acid producing bacteria and spoiled. A total of 102 strains not showing acid producing activity were isolated and characterized from spoiled cultures, and all the isolates were observed as Bacillus strains, including 64 strains (62.7%) and 16 strains (15.7%) of a Bacillus cereu s-related and B. fusiformis -related species, respectively. The Undaria substrate before fermentation contained culturable microorganisms at 1.4–3.1 × 102 CFU/g, but the Bacillus cereu s-related strain was not a major composition, suggesting a concern of selective growth of the Bacillus cereu s-related strain during the spoiled fermentation. |
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Keywords: | Bacillus cereus lactic acid fermentation Lactobacillus brevis Lactobacillus casei Lactobacillus plantarum Lactobacillus rhamnosus seaweed Undaria pinnatifida |
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