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| 湖南省鸡蛔虫线粒体nad1基因部分序列的测定及分析 | |
| 引用本文： | 张进,刘毅,邹建曾. 湖南省鸡蛔虫线粒体nad1基因部分序列的测定及分析[J]. 中国畜牧兽医, 2011, 38(2) |
| 作者姓名： | 张进 刘毅 邹建曾 |
| 作者单位： | 1. 湖南农业大学动物医学院,湖南长沙410128;长沙市动物防疫监督站,湖南长沙410013 2. 湖南农业大学动物医学院,湖南长沙,410128 3. 长沙市动物防疫监督站,湖南长沙,410013 |
| 摘 要： | 本研究旨在阐明鸡蛔虫湖南分离株线粒体烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位1(nad1)基因部分序列(pnad1)遗传变异情况。利用聚合酶链式反应(PCR)扩增鸡蛔虫的pnad1,应用Clustal X 1.81程序对序列进行比对。结果显示,所获得的pnad1序列长度一致,为408 bp。由于鸡蛔虫pnad1序列种内相对保守,种间差异较大,故可作为种间遗传变异研究的标记,从而为鸡蛔虫的分类鉴定及进一步的分子流行病学调查奠定了基础。 |
| 关 键 词： | 鸡蛔虫 线粒体DNA nad1基因 |
Sequence and Analysis of Partial Mitochondrial nad1 Gene for Ascaridia galli Isolates from Hunan Province |
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| ZHANG Jin,LIU Yi,ZOU Jian-zeng. Sequence and Analysis of Partial Mitochondrial nad1 Gene for Ascaridia galli Isolates from Hunan Province[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(2) | |
| Authors: | ZHANG Jin LIU Yi ZOU Jian-zeng |
| Affiliation: | ZHANG Jin1,2,LIU Yi1,ZOU Jian-zeng2(1.College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China,2.Supervision of Animal Epidemic Prevention Station of Hunan,Changsha 410013,China) |
| Abstract: | To analyze the differences of the NADH dehydrogenase subunit 1(nad1) gene of Ascaridia galli strains from Hunan province,the partial nad1(pnad1) was amplified from each Ascaridia galli sample,and pnad1 sequences were aligned using the Clustal X 1.81.The lengths of all the pnad1 sequences was 408 bp.There was no significant variation in pnad1 sequences within Ascaridia galli,while inter-species difference was obvious,so pnad1 sequences could be used as genetic marker for population genetic studies of roundwo... |
| Keywords: | Ascaridia galli mitochondrial DNA nad1 gene |
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