| 龙吐珠组培快繁技术初探 |
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| 引用本文： | 李鹤春,李成雁,庄金慧,李凤兰,胡国富. 龙吐珠组培快繁技术初探[J]. 安徽农业科学, 2009, 37(21): 9863-9866 |
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| 作者姓名： | 李鹤春 李成雁 庄金慧 李凤兰 胡国富 |
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| 作者单位： | 东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030 |
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| 摘 要： | [目的]探究龙吐珠最佳组织培养方案。[方法]以马鞭草科龙吐珠叶片、茎段、腋芽为外植体，进行组培快繁技术研究。[结果]最适宜外植体为茎段；最适宜消毒处理是消毒剂为0．1％升汞溶液，消毒时间为6min。产生侧芽的最佳培养基为1／2MS＋6-BA1．00mg／L＋NAA0．05ms／L＋IBA0．50mg／L，最适愈伤组织诱导及继代培养基为1／2MS＋6-BA2．00mg／L＋NAA0．10mg／L，平均诱导率为86．2％；最适分化培养基是1／2MS＋6-BA1．00mg／L＋NAA0．10mg／L＋KT 0．20mg／L，平均分化率为76．7％；不定芽的最适生根培养基是1／2MS＋N从0．01mg／L，其生根率为100％。[结论]该方案可为龙吐珠规模化生产提供技术支持。
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| 关 键 词： | 龙吐珠 组织培养 茎段 |
Discussion on the Tissue Culture and Rapid Propagation Techniques of Clerodendrum thomsonae Balf. |
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| LI He-chun et al. Discussion on the Tissue Culture and Rapid Propagation Techniques of Clerodendrum thomsonae Balf.[J]. Journal of Anhui Agricultural Sciences, 2009, 37(21): 9863-9866 |
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| Authors: | LI He-chun et al |
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| Affiliation: | LI He-chun et al (College of Life Science,Northeast Agricultural University,Harbin,Heilongjiang 150030) |
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| Abstract: | [Objective] This research aimed to find out the best tissue culture program of Clerodendrum thomsonae Balf.[Method] With the leaves,stem segments,axillary buds of C.thomsonae as explants,the tissue culture and rapid propagation techniques were studied.[Result] The optimum disinfection method was 0.1% solution of mercuric chloride as the disinfectant,and the disinfection course lasted 6 minutes.The best nutrient medium to produce lateral bud was 1/2 MS+1.00 mg/L 6-BA+0.05 mg/L NAA+0.50 mg/L IBA.The optimum c... |
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| Keywords: | Clerodendrum thomsonae Balf. Tissue culture Stem segments |
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