Lipid-rich bovine serum albumin improves the viability and hatching ability of porcine
blastocysts produced in vitro |
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Authors: | Chie SUZUKI Yosuke SAKAGUCHI Hiroyoshi HOSHI Koji YOSHIOKA |
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Affiliation: | 1)Pathology and Physiopathology Research Division, National Institute of Animal Health, Ibaraki 305-0856, Japan;2)Research Institute for the Functional Peptides, Yamagata 999-3766, Japan |
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Abstract: | The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts producedin vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium(PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching ratesof blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were culturedwith PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition ofLR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared withother treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 andDay-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts culturedwith PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymesinvolved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3,CPT1, CPT2 and KAT) in Day-7 blastocysts weresignificantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatchingability and quality of porcine blastocysts produced in vitro, as determined by ATP content,blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects ofLR-BSA arise from lipids bound to albumin. |
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Keywords: | ATP content Fatty acids Gene expression Pig embryo |
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