大豆顶芽和子叶节组织培养再生成苗的研究

以大豆品种小黑豆的顶芽作外植体,研究了激素、基本培养基对愈伤组织诱导及植株再生的影响.B_5+2,4-D0.5mg/L+KT0.1mg/L 作诱导培养基,愈伤组织诱导率达92.4%;愈伤组织在 MS+6-BA1mg/L+KT0.5mg/L+ZT0.5mg/L 的培养基上,分化频率47.2%.以子叶节为外植体,通过附加外源的6-BA 刺激子叶节处潜在的分生细胞,使其不断分裂,结果子叶节膨大增粗.切下子叶节培养,结果每个子叶节最高能分化出11棵正常的苗.

STUDY OF TISSUE CULTURE OF APICAL BUDS AND COTYLEDONARY NODES OF SOYBEAN

Using apical buds of Xiaoheidou as explants,the authors studied the effects ofhomones and basal media on the induction of calli and the regeneration of plants.The in-duction frequencey of calli on B_5+2,4-D 0.5 mg/L+KT 0.1 mg/L medium was 92.4%;When MS+6-BA 1 mg/L+KT 0.5 mg/L+ZT 0.5 mg/L was used as differentiationmedium,the differentiation frequency of buds reached as high as 47.2%.With cotyledonarynodes of Xiaoheidou as explants,by means of stimulating the cotyledonary nodes withhormone 6-BA,as many as 11 morphologically normal plants were obtained from the mul-tiple buds of each cotyledonary node.