| 大豆豆渣粗提物清除DPPH自由基活性及其协同效应的研究 |
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| 引用本文： | 葛飞,桂琳,陶玉贵,朱龙宝,黄寅.大豆豆渣粗提物清除DPPH自由基活性及其协同效应的研究[J].大豆科学,2010,29(1). |
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| 作者姓名： | 葛飞 桂琳 陶玉贵 朱龙宝 黄寅 |
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| 作者单位： | 安徽工程科技学院,生物化学工程系,安徽,芜湖,241000;微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000;皖南医学院,微生物和免疫学教研室,安徽,芜湖,241000;安徽工程科技学院,生物化学工程系,安徽,芜湖,241000 |
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| 基金项目： | 安徽省高校自然科学基金资助项目(KJ2009B071);;安徽工程科技学院引进人才科研启动基金资助项目(2007YQ005);;芜湖市科技计划资助项目(2008505) |
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| 摘 要： | 用二苯代苦味肼基自由基(DPPH)-TLC法和酶标仪法对大豆豆渣石油醚、乙酸乙酯、丙酮、乙醇粗提物的自由基清除活性进行了定性和定量分析,考察了抗氧化剂维生素C、柠檬酸对大豆豆渣乙醇粗提物清除DPPH自由基活性的协同效应。结果表明:大豆豆渣不同极性粗提物均有一定的自由基清除活性,其中乙醇粗提物的自由基清除活性最强,在浓度为10.0 mg.mL-1,于37℃下保温15 min时,对0.4 mg.mL-1的DPPH自由基的清除率可达76.48%;维生素C和柠檬酸对大豆豆渣乙醇粗提物均能产生一定的协同效应,且维生素C的协同作用强于柠檬酸。
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| 关 键 词： | 豆渣提取物 DPPH 抗氧化 协同作用 |
DPPH Radical Scavenging Activity of Extract from Soybean Residue and Coordination Effect |
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| GE Fei,GUI Lin,TAO Yu-gui,ZHU Long-bao,HUANG Yin.DPPH Radical Scavenging Activity of Extract from Soybean Residue and Coordination Effect[J].Soybean Science,2010,29(1). |
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| Authors: | GE Fei GUI Lin TAO Yu-gui ZHU Long-bao HUANG Yin |
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| Institution: | 1.Department of Biochemical Engineering;Anhui University of Science and Technology;Wuhu 241000;2.Anhui Engineering Technology Research Center of Microbial Fermentation;3.Department of Microbiology and immunology;Wannan Medical College;Anhui;China |
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| Abstract: | To study the DPPH radical scavenging activity of the extracts from soybean residue and the coordination effect with other antioxidants,DPPH-TLC and DPPH-Microplate assays were used to determine the free radical scavenging activities of the petroleum ether,ethyl acetate,acetone and ethanol extracts from soybean residue.The results revealed that these extracts all had free radical scavenging activities.The DPPH radical scavenging activity of the ethanol extracts was much higher than that of petroleum ether,et... |
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| Keywords: | DPPH Extract from soybean residue 1-Dphenyl-2-picrylhydrazyl(DPPH) Antioxidation effect Coordination effect |
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