| 小麦抗叶锈病基因Lr2c的SSR标记 |
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| 引用本文： | 张娜,闫红飞,张英春,李爱霞,陈玉婷,张立荣,杨文香,刘大群. 小麦抗叶锈病基因Lr2c的SSR标记[J]. 农业生物技术学报, 2009, 17(1): 148-152 |
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| 作者姓名： | 张娜 闫红飞 张英春 李爱霞 陈玉婷 张立荣 杨文香 刘大群 |
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| 作者单位： | 河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,保定071001 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金 |
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| 摘 要： | 选取抗叶锈病基因位于2D染色体上的TcLr2c等7个小麦（Triticum aestivum）近等基因系、感病亲本Thatcher及215株TcLr2c与Thatcher杂交F2代为材料，研究抗叶锈病基因Lr2c SSR分子标记。从筛选的29对位于小麦2D染色体的SSR引物中获得4对能够揭示Lr2c多态性的分子标记，通过215株TcLr2c × Thatcher F2群体验证，结果表明Xgwm261和Xgwm296与Lr2c紧密连锁，其距目的基因的遗传距离分别为1.9和3.6 cM，可用于小麦抗叶锈病分子辅助育种。
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| 关 键 词： | 小麦抗叶锈病基因；Lr2c；SSR；分子标记 |
| 收稿时间： | 2008-04-07 |
| 修稿时间： | 2008-05-15 |
SSR Markers Linked to Leaf Rust Resistance Gene Lr2c in Wheat |
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| ZHANG Na,YAN Hong-fei,ZHANG Ying-chun,LI Ai-Xia,CHEN Yu-ting,ZHANG Li-rong,YANG Wen-xiang,LIU Da-qun. SSR Markers Linked to Leaf Rust Resistance Gene Lr2c in Wheat[J]. Journal of Agricultural Biotechnology, 2009, 17(1): 148-152 |
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| Authors: | ZHANG Na YAN Hong-fei ZHANG Ying-chun LI Ai-Xia CHEN Yu-ting ZHANG Li-rong YANG Wen-xiang LIU Da-qun |
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| Abstract: | SSR analysis was performed based on 7 wheat (Triticum aestivum) near iso-genic lines (NILs) with Lr genes located on chromosome 2D including two multiple alleles of Lr2c, the susceptible control Thatcher, and a population of 215 F2 plants derived from TcLr2c × Thatcher, respectively to identify the SSR marker linked to wheat leaf rust resistance gene Lr2c. Twenty-nine SSR primer pairs on wheat 2D chromosome were screened and four of them could generate polymorphisms to the other near isogenic lines. Result showed that Xgwm261 and Xgwm296 were tightly linked to the gene Lr2c with a distance of 1.9 and 3.6 cM respectively using test and verify of the population of 215 F2 derived from TcLr2c × Thatcher, which could be used in molecular assisted breeding against wheat leaf rust. |
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| Keywords: | Lr2c SSR |
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