| 半定量检测新城疫抗体胶体金试纸条的研制 |
| |
| 引用本文： | 李希友,田夫林,张秀娥,李建亮,崔言顺. 半定量检测新城疫抗体胶体金试纸条的研制[J]. 畜牧兽医学报, 2006, 37(12): 1373-1377 |
| |
| 作者姓名： | 李希友 田夫林 张秀娥 李建亮 崔言顺 |
| |
| 作者单位： | 1. 山东农业大学动物医学院,泰安,271018
2. 山东农业大学动物医学院,泰安,271018;山东省畜牧兽医总站检验技术中心,济南,250022 |
| |
| 摘 要： | 为建立半定量快速检测新城疫抗体方法，本研究利用柠檬酸钠还原氯金酸，形成胶体金颗粒，应用直径20nm的胶体金颗粒标记新城疫抗原，制作了半定量快速测定新城疫抗体的免疫胶体金试纸条。用试纸条检测60份样品，检测结果与血凝抑制试验（HI）法测得结果的符合率为93．8％，最小检测HI滴度为410g2，与其他病毒的阳性血清没有交叉反应，且室温下有效保存期为3个月。试纸条应用方便、快速，适合临床推广以及流行病学调查。
|
| 关 键 词： | 新城疫 胶体金 抗体 试纸条 |
| 文章编号： | 0366-6964（2006）12-1373-05 |
| 收稿时间： | 2006-03-29 |
| 修稿时间： | 2006-03-29 |
Preparation of Colloidal Gold Strip for Semi-quantitative Test of Antibody against Newcastle Disease Virus |
| |
| LI Xi-you,TIAN Fu-lin,ZHANG Xiu-e,LI Jian-liang,CUI Yan-shun. Preparation of Colloidal Gold Strip for Semi-quantitative Test of Antibody against Newcastle Disease Virus[J]. Chinese Journal of Animal and Veterinary Sciences, 2006, 37(12): 1373-1377 |
| |
| Authors: | LI Xi-you TIAN Fu-lin ZHANG Xiu-e LI Jian-liang CUI Yan-shun |
| |
| Abstract: | To develop a "semi-quantitative RUN test" method to detect antibody against Newcastle disease virus,Tri-sodium citrate with aqueous gold chloride were warmed up and mixed to make gold sol.The 20nm-diameter colloidal gold was used to mark NDV antigen and the colloidal gold strip was prepared for semi-quantitative test of antibody against NDV.60 samples were tested by the colloidal gold strip.The coincidence between the colloidal gold strip and HI was 93.8%.The least test level of the strip was 4log2 detected by HI experiment,and it didn't exist cross-reaction with other serums against corresponding virus.The strip was still effectual after storing three months at room temperature.Colloidal gold kit is a rapid method in clinic practice and could be used in surveillance of antibody against NDV. |
| |
| Keywords: | Newcastle disease colloidal gold antibody strip |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
