| 玉米中黄曲霉毒素提取方法的评价 |
| |
| 引用本文： | 韩红岩,汪军.玉米中黄曲霉毒素提取方法的评价[J].山东农业大学学报(自然科学版),1999,30(3):241-244. |
| |
| 作者姓名： | 韩红岩 汪军 |
| |
| 作者单位： | 山东农业大学遗传所 |
| |
| 摘 要： | 用多种溶剂与玉米的不同比例对玉米中黄曲霉毒素 Ｂ１ 的提取效率进行评价。结果发现，用６０ ％ 甲醇水溶液为提取剂，与样品比例为６ ：１（ ｍｌ／ｇ） ；８０ ％ 丙酮水溶液，与样品比例为５ ：１ ，６ ：１（ ｍｌ／ｇ） 及９０ ％ 乙腈水溶液，与样品比例为４ ：１（ ｍｌ／ｇ） 的提取效果较好。
|
| 关 键 词： | 玉米 黄曲霉毒素 |
EVALUATION OF EXTRACTION METHODS OF AFB 1 IN CORN |
| |
| Han Hongyan,Wang Jun.EVALUATION OF EXTRACTION METHODS OF AFB 1 IN CORN[J].Journal of Shandong Agricultural University,1999,30(3):241-244. |
| |
| Authors: | Han Hongyan Wang Jun |
| |
| Abstract: | A study was conducted to compare the extraction efficients of aflatoxins B 1 from naturally contaminated corn with different solvents and solvent/corn ratios.The results show that better extraction efficients were found by using the following solvents:60% methanol/water at 6:1 (ml/g) solvent to sample ratio;80% acetone/water at 5:1,6:1(ml/g)solvent to sample ratio and 90% acetonitrile/water at 4:1(ml/g)solvent to sample ratio.Extraction liquid was treated with petroleum ether to raze the fat,extracted by using chloroform and concentrated by reducing pressure.The AFB 1 was determined with high efficient liquid chromatography(LC). |
| |
| Keywords: | corn aflatoxin B 1 extraction method |
| 本文献已被 CNKI 维普 等数据库收录！ |
