SRAP marker reveals genetic diversity in tartary buckwheat in China |
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Authors: | Yanqin Li Xiaoling Fan Tonglin Shi Quanbin Zhang Zongwen Zhang |
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Institution: | (1) Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada |
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Abstract: | Sequence-related amplified polymorphism (SRAP) marker was employed to analyze genetic diversity of 10 accessions of tartary
buckwheat selected from a wide geographical area in China. Of the total 30 primer combinations investigated, 26 could amplify
clearly and consistently. They produced a total of 285 fragments, of which 235 (82.5%) were polymorphic bands. Among the 26
primer combinations, five could discriminate all the genotypes used in this study. Based on the molecular data, the genetic
similarity coefficients varied from 0.61 to 0.78 and calculated using the NTSYSpc published by Nei and Li (1979). The cluster
analysis revealed that the 10 accessions were better to be grouped into two major clusters at a similarity level of 0.69.
Moreover, the accessions collected from the same province turned out to be grouped in the same cluster, which indicated some
geographical relationships. It also proved that the SRAP marker system was useful in identification and genetic diversity
analysis of tartary buckwheat. |
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Keywords: | |
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