田间枣树愈伤组织诱导及不定芽的发生

以‘星光'和‘月光'2个枣树品种为试材,比较了截干高度和截口粗度、覆盖方式、二甲基亚砜(DMSO)、TDZ(N-苯基-N'-1,2,3-噻二唑-5-基脲)、AgNO3和秋水仙素对田间枝干截面愈伤组织诱导和不定芽发生的影响.结果表明:高枝干截面愈伤发生快,愈伤生长旺盛,截口粗度对截面愈伤的发生有影响,差异显著.“塑料袋+...

Callus induction and adventitious bud occurrence from Chinese jujube in the field

Effect of stumping height and cutting diameter of branches,covering types,dimethyl sulfoxide(DMSO),TDZ(Thidiazuron),AgNO3 and colchicine on callus induction and adventitious occurrence from cross section of Chinese jujube in the field was investigated by using two varieties of Zizyphus jujube Mill.cv.'Xingguang'and 'Yueguang'.The results showed callus from cross section of higher branches formed early and developed quickly.Callus formation varied significantly with branch cutting diameter.'Plastic bag+ mud + plastic bag' was the optimal covering type for callus induction and adventitious bud occurrence on cross section of branches,by which callus occurred so rapidly that transparent callus was visible in 5 days.Finally callus rate was as high as 100% and adventitious bud rate reached 80%.Callus formed lately when 2% DMSO was added.4.0 mg/L TDZ+2.0 mg/L AgNO3 did not obviously accelerate callus induction and adventitious bud formation.However,adventitious bud was hindered by colchicines.0.025% colchicines caused the least rate of adventitious bud in 'Xingguang'and 'Yueguang' and the value was separately 45% and 5%.Compared to 'Yueguang',callus appeared early on branches cross section of 'Xingguang' and its adventitious bud rate was high.So,the chromosome mutagenesis was more probably achieved in 'Xingguang' than 'Yueguang'.