| 貉GAPDH基因的克隆与序列分析 |
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| 引用本文： | 侯志军,张长生,金辛. 貉GAPDH基因的克隆与序列分析[J]. 经济动物学报, 2012, 16(1): 35-37 |
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| 作者姓名： | 侯志军 张长生 金辛 |
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| 作者单位： | 东北林业大学野生动物资源学院,哈尔滨,150040 |
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| 基金项目： | 中央高校基本科研业务费专项基金，黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 根据哺乳动物的甘油醛-3-磷酸脱氢酶(glycera ldehyde-3-phosphate dehydrogenase,GAPDH)氨基酸的保守序列,参考犬的GAPDH基因序列,设计一对特异性引物,采用RT-PCR技术,从貉的肝脏中扩增出一段长271 bp的序列。对其序列分析表明:该基因序列与GenBank中已发表的犬的GAPDH基因(GenBank序列号:AB028142,AB038240)同源性为100%。本试验成功地克隆了一段貉GAPDH基因,证实了貉亦存在GAPDH基因且高度保守。
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| 关 键 词： | 貉 GAPDH甘油醛-3-磷酸脱氢酶 克隆 |
Cloning and Sequencing of Raccoon Dog GAPDH Gene |
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| HOU Zhi-jun,ZHANG Chang-sheng,JIN Xin. Cloning and Sequencing of Raccoon Dog GAPDH Gene[J]. Journal of Economic Animal, 2012, 16(1): 35-37 |
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| Authors: | HOU Zhi-jun ZHANG Chang-sheng JIN Xin |
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| Affiliation: | (College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | The Raccoon dog GAPDH gene was amplified and cloned by RT-PCR from its liver.The gene was sequenced by 271 bp.Compared with the genes of canine(GenBank numbers:AB028142,AB038240),they shared 100% homology.This trial proved that the GAPDH gene was existed and conserved in raccoon dog. |
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| Keywords: | raccoon dog GAPDH clone |
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