快速估测反刍动物瘤胃微生物蛋白在小肠流量的方法

选用瘤胃尚未发育的初生小尾寒羊羔羊及梅花仔鹿各4只,人工哺乳,随牛奶分别饲喂给羔羊和仔鹿5个水平的核酸。羔羊尿中嘌呤衍生物的总量(Y,mmol/d)随着摄入嘌呤量(X,mmol/d)的增加而增加。其线性方程为:Y=0.752+0.662X(r=0.995,P<0.001)。仔鹿尿中嘌呤衍生物的总量(Y,mmol/d)随着摄入嘌呤量(X,mmol/d)的增加也明显增加,线性方程为:Y=0.559+0.326X(r=0.990,P=0.001)。利用紫外分光光度计测定的羔羊尿中尿酸当量值与嘌呤摄入量呈强正相关(r=0.994,P<0.01)。由此推算出利用尿中尿酸当量值来估测羊小肠微生物氮流量(Y,g/d)的方程为:Y=0.417+0.396X;推算出的利用尿中尿酸当量值估测鹿小肠微生物氮流量(Y,g/d)的方程为:Y=-4.137+1.245X。X(mmol/d)为测得的羊或鹿尿中尿酸当量值。

A Simple Method for Estimating Microbial Protein Flow to the Small Intestine of Ruminants

Four pre-rumen lambs and four fawns were used to study nucleic acid metabolism.Nucleic acids dissolved in milk were fed to the animals at 5 levels.Total purine derivative excretion(including allantoin,uric acid,hypoxanthine and xanthine)(Y,mmol/d) in the urine increased with increasing exogenous purine inputs(X,mmol/d) by lambs,its linear equation was: Y=0.752+0.662X(r=0.995,P<0.001).Total purine derivative excretion(Y,mmol/d) in the urine by fawns increased with increasing exogenous purine inputs(X,mmol/d),its linear equation was: Y=0.559+0.326X(r=0.990,P<0.01).Uric acid equivalents(Y,mmol/d) determined by using spectrophotometer in the urine of lambs was closely related with exogenous purine inputs(r=0.994,P<0.01).The predicted equation for the estimation of microbial nitrogen flow(Y,g/d) in the duodenum of sheep using uric acid equivalents was: microbial nitrogen flow Y=0.417+0.396X.Similarly,the predicted equation for the estimation of microbial nitrogen flow(Y,g/d) in the duodenum of deer using the uric acid equivalents was: microbial nitrogen flow Y=-4.137+1.245 X.The X(mmol/d) in the above two equations was the uric acid equivalents in the urine of lamb or fawns.