猴头菌醋糟高产配方筛选及营养利用规律研究

本研究运用五元二次正交旋转回归组合设计进行了糟栽培猴头菌高产配方的初步筛选，并对猴头菌生育期间营养利用规律及酶活变化规律进行了探讨。结果表明，醋糟配料中的麸皮，黄豆粉和过磷酸钙与配料中的其它辅料通过互作产生了增产效应。经优化筛选，获得醋糟栽培猴头菌的两种高产配方：（1）醋糟44．12％，锯末25％，麦麸20％，玉米粉0．63％，黄豆粉5％，过磷酸钙2．25％，石膏2％，白糖1％；（2）醋糟43．50％，锯末30％，麦麸11．25％，玉米粉5％，黄豆粉5％，过磷酸钙2．25％，石膏2％，白糖1％。猴头菌菌丝生长阶段主要降解木质素，而子实体生长阶段主要降解纤维素和半纤维素，且漆酶，愈创木酚氧化酶，纤维素酶，半纤维素酶酶活性的变化与木质素，纤维素及半纤维素的降解规律一致。

A Study on the Screening of High-Yield Formulae of Vinegar Distillers' Grain and Nutritional Utilization Law for Hericium erinaceus

The quadratic orthogonal rotational regression combination design with five factors was used to screen the high yield formulae of vinegar distillers' grain compost for Hericium erinaceus in this paper. And the utilization law of nutritional components and the change law of extracellular enzyme activity during the whole growth period of H. erinaceus were investigated too. The results showed that the yield-increment effect was produced through the interaction of wheat bran, soybean powder and superphosphate in the vinegar distillers' grain with other subsidiary components. And it was proved in our optimization screening that the formulae of vinegar distillers' grain 44.12%, sawdust 25%, wheat bran 20%, corn meal 0.63%, soybean powder 5%, superphosphate 2.25%, gypsum 2% and sugar 1%, or vinegar distillers' grain 43.50%, sawdust 30%, wheat bran 11.25%, corn meal 5%, soybean powder 5%, superphosphate 2.25%, gypsum 2% and sugar 1% were two optimum formulae. While it was also confirmed in our test that lignin was mainly decomposed during the vegetative growth period of H. erinaceus, and cellulose and hemicellulose were mainly decomposed during the reproductive growth period of H. erinaceus. And the change law of laccase, guaiacol oxidase, cellulase and hemicellulase activity was identical with the decomposition law of lignin, cellulose and hemicellulose.