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黄绿蜜环菌菌丝多肽分离的初步研究
引用本文:焦迎春,杨春江,周劲松,熊辉岩,盛海彦.黄绿蜜环菌菌丝多肽分离的初步研究[J].农产品加工.学刊,2008(6):8-11.
作者姓名:焦迎春  杨春江  周劲松  熊辉岩  盛海彦
作者单位:1. 青海大学,生物科学系,青海,西宁,810016
2. 青海大学,农牧学院农林系,青海,西宁,810016
3. 浙江建筑职业技术学院,浙江,杭州,311231
摘    要:分别采用体积分数为60%,70%,80%,90%的乙醇,对黄绿蜜环菌菌丝粉中的多肽进行提取,通过Sephadex G-75和Sephadex G-25凝胶二次层析分离后,对4种不同乙醇体积分数的提取液进行比较,结果显示,采用体积分数为70%的乙醇的提取效果较好。同时毛细管电泳的结果也表明,体积分数为70%的乙醇提取液中有较多均匀分布的小肽。

关 键 词:黄绿蜜环菌菌丝肽  凝胶层析  毛细管电泳

Initial Studies on the Separation and Analysis of Mycelium Peptides in Armillaria Luteo-virens
Jiao Yingchun,Yang Chunjiang,Zhou Jinsong,Xiong Huiyan,Sheng Haiyan.Initial Studies on the Separation and Analysis of Mycelium Peptides in Armillaria Luteo-virens[J].Nongchanpin Jlagong.Xuekan,2008(6):8-11.
Authors:Jiao Yingchun  Yang Chunjiang  Zhou Jinsong  Xiong Huiyan  Sheng Haiyan
Institution:Jiao Yingchun1,Yang Chunjiang2,Zhou Jinsong3,Xiong Huiyan2,Sheng Haiyan2(1.Department of Biological Sciences,Qinghai University,Xining,Qinghai 810016,China,2.Department of Farming , Forestry,3.Zhejiang College of Construction,Hangzhou,Zhejiang 311231,China)
Abstract:The peptides in armillaria luteo-virens mycelium were extracted at four different ethanol concentration60%,70%,80%,90%,then the extracted peptides were twice isolated with sephadex G-75 and sephadex G-25 gelchromatography preliminarily.The comparison of the extractive results of four different ethanol concentrations showed that 70% were better.The results of capillary electrophoresis showed that 70% ethanol extracted liquid contained many peptides.
Keywords:mycelium peptides of armillaria luteo-virens  gelchromatography  capillary electrophoresis  
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