Cryopreservation of Sheep Primordial Follicles |
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| Authors: | CA Amorim,D Rondina,CM Lucci,A Giorgetti,JR de Figueiredo, PBD Gonç alves |
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| Affiliation: | Programa de Pós-graduação em Medicina Veterinária, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil;;Programa de Pós-graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil;;Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Brasília, DF, Brazil;;Dipartimento di Scienze Zootecniche, Universitàdegli Studi di Firenze, Florence, Italy |
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| Abstract: | The aim of this study was to evaluate the efficiency of 1 M dimethylsulphoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH) and glycerol (GLY) to cryopreserve primordial follicles. The first evaluation was performed soon after cryopreservation and the second evaluation after 4 days of in vitro culture, using the cryoprotectants that allowed the higher results (higher follicular survival rate) after cryopreservation. The results after follicular isolation (control) and cryopreservation using 1 M DMSO, EG, PROH and GLY showed that the mean number (+/- SEM) of live follicles per millilitre was 3204 (100%) +/- 319.27, 2798 (87%) +/- 239.14, 2492 (78%) +/- 345.8, 448 (14%) +/- 46.3 and 208 (7%) +/- 75.26, respectively. Higher follicular survival was reported when DMSO and EG were used. Control follicles and follicles cryopreserved with these two cryoprotectants were cultured and the percentage of follicular survival was 55% (control), 42% (EG) and 34% (DMSO). Similar results were found between control and follicles cryopreserved with EG. In conclusion, 1 M EG is the most effective cryoprotectant to preserve primordial follicles isolated from ovaries of sheep. |
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