Influence of different cellular concentrations of boar sperm suspensions on the induction of capacitation and acrosome reaction |
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| Authors: | David MARTÍN-HIDALGO Beatriz MACÍAS-GARCÍA Lauro GONZLEZ-FERNNDEZ |
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| Institution: | 1)Research Group of Intracellular Signalling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, Spain;2)Department of Biochemistry and Molecular Biology and Genetics, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain;3)Department of Animal Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain |
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| Abstract: | We aimed to analyze the influence of different cellular concentrations of boar sperm suspensions on the induction of capacitation and acrosome reaction. When spermatozoa were incubated at 100 or 200 mill/ml, significant increases in protein tyrosine phosphorylation in the p32 protein were observed, compared to those at 50 mill/ml. In addition, sperm concentration-dependent increases were observed in plasma membrane lipid disorganization (50 mill/ml vs. 200 mill/ml), induction of the acrosome reaction (50 mill/ml vs. 100 mill/ml and 200 mill/ml), and sperm viability (50 mill/ml vs. 100 mill/ml and 200 mill/ml). Our data indicate that an increase in sperm concentration stimulates the induction of capacitation and acrosome reaction in boars. |
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| Keywords: | Acrosome reaction Boar spermatozoa Capacitation |
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