Ligiamycins A and B,Decalin-Amino-Maleimides from the Co-Culture of Streptomyces sp. and Achromobacter sp. Isolated from the Marine Wharf Roach,Ligia exotica |
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Authors: | Hyung-Ju Lim Joon Soo An Eun Seo Bae Eunji Cho Sunghoon Hwang Sang-Jip Nam Ki-Bong Oh Sang Kook Lee Dong-Chan Oh |
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Institution: | 1.Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea; (H.-J.L.); (J.S.A.); (E.S.B.); (S.H.); (S.K.L.);2.Department of Agriculture Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea; (E.C.); (K.-B.O.);3.Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Korea; |
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Abstract: | Streptomyces sp. GET02.ST and Achromobacter sp. GET02.AC were isolated together from the gut of the wharf roach, Ligia exotica, inhabiting the intertidal zone of the west coast of Korea. The co-cultivation of these two strains significantly induced the production of two new metabolites, ligiamycins A (1) and B (2), which were barely detected in the single culture of Streptomyces sp. GET02.ST. The planar structures of ligiamycins A (1) and B (2) were elucidated as new decalins coupled with amino-maleimides by the analysis of various spectroscopic data, including nuclear magnetic resonance (NMR), ultraviolet (UV), and mass (MS) data. The assignment of two nitrogen atoms in amino-maleimide in 1 was accomplished based on 1H-15N heteroatom single quantum coherence spectroscopy (HSQC) NMR experiments. The relative configurations of the ligiamycins were determined using rotating frame Overhauser effect spectroscopy (ROESY) NMR data, and their absolute configurations were deduced by comparing their experimental and calculated optical rotations. Ligiamycin A (1) displayed antibacterial effects against Staphylococcus aureus and Salmonella enterica, while ligiamycin B (2) exhibited mild cell cytotoxicity against human colorectal cancer cells. |
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Keywords: | co-culture Streptomyces Achromobacter structure elucidation Ligia exotica optical rotation cytotoxicity antibacterial decalin maleimide |
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