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| 花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效 | |
| 引用本文： | 王小兵,骆永明,刘五星,李振高.花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效[J].中国生物防治,2011,27(1):88-92. |
| 作者姓名： | 王小兵 骆永明 刘五星 李振高 |
| 作者单位： | 1. 中国科学院土壤环境与污染修复重点实验室,南京土壤研究所,南京,210008;中国科学院研究生院,北京,100049;扬州大学环境科学与工程学院,扬州,225009 2. 中国科学院土壤环境与污染修复重点实验室,南京土壤研究所,南京,210008;中国科学院研究生院,北京,100049 3. 中国科学院土壤环境与污染修复重点实验室,南京土壤研究所,南京,210008 |
| 基金项目： | 国家自然科学基金重点项目(40432005); 中国科学院知识创新工程重要方向项目(CXTD-Z2005-4) |
| 摘 要： | 从病区健康花生植株茎秆内分离到1株对花生青枯病菌Ralstonia solanacearum有强拮抗作用的内生细菌,命名为BZ6-1菌株.拮抗试验表明,该菌具有较广抗菌谱,对多种植物病原真菌有较强的抑制作用.形态观察和16S rDNA同源性分析初步鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens.该菌的最佳培养基组成为:糖蜜10g·L-1,蛋白胨0.5g·L-1,酵母膏0.5g·L-1.最适发酵条件为pH8.5、装液量50mL/250mL、温度25℃、转速200r·min-1、培养时间21h,在此条件下拮抗圈直径达34.0mm.田间试验结果表明BZ6-1菌株对花生青枯病防效达62.3%. |
| 关 键 词： | 花生青枯病 解淀粉芽孢杆菌 内生细菌 抗菌活性 田间防效 |
Identification,Antimicrobial Activity and Field Control Efficacy of an Endophytic Bacteria Strain against Peanut Bacterial Wilt |
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| WANG Xiao-bing , LUO Yong-ming , LIU Wu-xing , LI Zhen-gao.Identification,Antimicrobial Activity and Field Control Efficacy of an Endophytic Bacteria Strain against Peanut Bacterial Wilt[J].Chinese Journal of Biological Control,2011,27(1):88-92. | |
| Authors: | WANG Xiao-bing LUO Yong-ming LIU Wu-xing LI Zhen-gao |
| Institution: | WANG Xiao-bing1,2,3,LUO Yong-ming1,LIU Wu-xing1,LI Zhengao1(1.Key Laboratory of Soil Environment and Pollution Remediation,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,2.Graduate School of the Chinese Academy of Sciences,Beijing 100049,3.College of Environmental Sciences and Engineering,Yangzhou University,Yangzhou 225009,China) |
| Abstract: | An endophytic bacteria strain BZ6-1,isolated from the stem of healthy peanut plants in Ralstonia solanacearum-infested fields,suppressed R.solanacearum greatly and was of broad spectrum against plant pathogenic fungi.According to its morphology and 16S rDNA sequence,the strain BZ6-1 was preliminarily identified as Bacillus amyloliquefaciens.Diameter of inhibition zone reached 34.0 mm under the optimal fermentation conditions for antibiotic production,which was molasses 10 g,peptone 0.5 g and yeast extract 0... |
| Keywords: | peanut bacterial wilt Bacillus amyloliquefaciens endophytic bacteria antimicrobial activity field control efficacy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |