| 快速诊断伪狂犬病毒的LAMP方法的建立 |
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| 引用本文： | 杨睿,杨金龙,王孝友,付利芝,曾秀. 快速诊断伪狂犬病毒的LAMP方法的建立[J]. 中国兽医学报, 2011, 31(2) |
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| 作者姓名： | 杨睿 杨金龙 王孝友 付利芝 曾秀 |
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| 作者单位： | 重庆市畜牧科学院,重庆,荣昌,402460 |
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| 基金项目： | 重庆市科技攻关项目(CSTC,2009AC1133); 十一五国家科技支撑计划子课题(2007BAD51B05-2); 重庆市畜牧科学院科技攻关计划(09602) |
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| 摘 要： | 采用LAMP技术建立了一种针对伪狂犬病毒的可视、快速、灵敏、特异的检测方法。应用PrimerExplorerV4软件,根据伪狂犬gE基因的保守序列设计了4条引物,优化了反应体系与反应时间,检测了特异性与灵敏性。发现该方法只需要40 min就能检测出结果,具有良好的特异性,灵敏度为普通PCR的100倍,最低可检测出质量浓度为6.2×10-9mg/L的病毒DNA。该方法的建立为伪狂犬病的快速诊断提供了新的思路。
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| 关 键 词： | LAMP 伪狂犬病毒 PCR |
Establishment of a rapid method for detection of pseudorabies virus by a loop-mediated isothermal amplification |
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| YANG Rui,YANG Jing-long,WANG Xiao-you,FU Li-zhi,ZENG Xiu. Establishment of a rapid method for detection of pseudorabies virus by a loop-mediated isothermal amplification[J]. Chinese Journal of Veterinary Science, 2011, 31(2) |
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| Authors: | YANG Rui YANG Jing-long WANG Xiao-you FU Li-zhi ZENG Xiu |
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| Affiliation: | YANG Rui,YANG Jing-long,WANG Xiao-you,FU Li-zhi,ZENG Xiu(Chongqing Academy of Animal Sciences,Rongchang,Chongqing 402460,China) |
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| Abstract: | A loop-mediated isothermal amplification(LAMP) assay was developed for visible,rapid and sensitive detection of pseudorabies virus(PRV).A set of four primers were designed based on the PRV gE gene sequence using PrimerExplorerV4 software,optimizing reaction system and reaction time and detecting specificity and sensitivity.It is shown that the virus could be detected within 40 minutes by the method,with ideal specificity,and the sensitivity was 100 times higher than that of general PCR,and the detection lim... |
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| Keywords: | loop-mediated isothermal amplification pseudorabies virus polymerase chain reaction |
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